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Rabbit anti VEGF Polyclonal

VEGF

accession:  P15692
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA15692
Applications:
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
VEGF Immunohistochemistry/IHC

Immunohistochemical analysis of PPFE mouse Ovrian tissue sections expressing VEGF.

Cat# RPA15692 was used as primary antibody, dilution 1:500, DAB chromogenic staining.

 

 

 

VEGF Immunohistochemistry/IHC

Immunohistochemical analysis of PPFE Dorsal skin tissue sections expressing VEGF.

Cat# RPA15692 was used as primary antibody, dilution 1:500, DAB chromogenic staining.

 

 

 

 
Source:
Polyclonal VEGF antibody was produced from sera of  rabbits immunized with synthetic protein containing VEGF antigenic determinants. 
 
Species reactivity:
Human,Mouse,Rat,Cow,Sheep,Pig
 
Purification:

Serum IgG fraction was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

VEGF

VEGF is a homodimeric heavily glycosylated protein.The human factor occurs in several molecular variants of 121, 162, 145, 148, 165, 183, 189 , 206 amino acids, arising by alternative splicing of the mRNA.The splice forms of VEGF differ in biological properties such as the receptor types, which they recognize and their interaction with heparan sulfate proteoglycans. The 165 amino acid form of the factor is the most common form in most tissues. Kaposi sarcomas express VEGF121 and VEGF165. VEGF121 and VEGF165 are soluble secreted forms of the factor while VEGF189 and VEGF206 are mostly bound to heparin-containing proteoglycans in the cell surface or in the basement membrane. A high-affinity glycoprotein receptor of 170-235 kDa is expressed on vascular endothelial cells. The interaction of VEGF with heparin-like molecules of the extracellular matrix is required for efficient receptor binding. Protamine sulfate and suramin are capable of replacing the receptor-bound factor. The high-affinity receptor for VEGF, now known as VEGFR1, has been identified as the gene product of the FLT-1. Another receptor for VEGF, now known as VEGFR2, is KDR, also known as FLK-1. A factor that competes with the 165 amino acid form of VEGF for receptor binding is PLGF. A third receptor type, VEGFR3 is known also as FLT- 4. An isoform-specific receptor for VEGF165 has been identified as human Neuropilin-1.

Related Publications:
age-associated differences in activation of akt/gsk-3β signaling pathways and inhibition of mitochondrial permeability transition pore opening in the rat heart
J Gerontol A Biol Sci Med Sci, Jun 2010; 65A: 611 - 619.
troglitazone ameliorates high glucose-induced emt and dysfunction of sglts through pi3k/akt, gsk-3β, snail1, and β-catenin in renal proximal tubule cells
Am J Physiol Renal Physiol, May 2010; 298: F1263 - F1275.
hydrogen sulfide protects cardiomyocytes from hypoxia/reoxygenation-induced apoptosis by preventing gsk-3β-dependent opening of mptp
Am J Physiol Heart Circ Physiol, May 2010; 298: H1310 - H1319.
troglitazone ameliorates high glucose-induced emt and dysfunction of sglts through pi3k/akt, gsk-3β, snail1, and β-catenin in renal proximal tubule cells
Am J Physiol Renal Physiol, May 2010; 298: F1263 - F1275.
extracellular ubiquitin inhibits β-ar-stimulated apoptosis in cardiac myocytes: role of gsk-3β and mitochondrial pathways
Cardiovasc Res, Apr 2010; 86: 20 - 28.




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Antibodies:
Purified Polyclonal
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