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Rabbit anti NK1(Substance-P) Polyclonal

Neurokinin-1

accession:  P20366
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA20366
Applications:
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
NK1 IHC

Immunohistochemical analysis of Neurokinin-1 expression in FFPE mouse pancreatic islets.

Cat#RPA20366 was used as primary antibody, dilution 1:400.DAB chromogen substrate.

NK1 IHC

Immunohistochemical analysis of Neurokinin-1 expression in rat colon tissue.

Cat#RPA20366 was used as primary antibody, dilution 1:500, DAB chromogen substrate.

 
Source:
Polyclonal neurokinin-1 (Substance-P) antibody was produced from sera of rabbits  immunized with synthetic protein containing neurokinin-1  antigenic determinants. 
 
Species reactivity:
Human,Mouse,Rat
 
Purification:

Serum IgG fraction was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Neurokinin-1

NK-1, an 11-amino-acid neuropeptide, is released from enteric nerves, sensory neurons, and inflammatory cells of the lamina propria during intestinal inflammation. Conversely, Substance-P activates nerves, epithelial cells, and a variety of immune and inflammatory cells, leading to the release of cytokines, chemokines, and other neuropeptides that modulate diarrhea, inflammation, and motility changes associated with several intestinal disease states, including IBD . Substance-P mediates the development and progression of intestinal inflammation by binding to its high-affinity receptor, the neurokinin 1 receptor (NK-1R). Our laboratory has shown that SP-NK-1R interactions play a dual role in the development and maintenance of colonic inflammation. Thus NK-1R- deficient mice are protected during the acute phase of colitis yet exhibit an enhanced inflammatory response during chronic colitis. Neurokinin-1 was also recently shown to rescue colonic cells from apoptosis, preserving colonic tissue integrity in a murine DSS colitis model

Related Publications:
tgf-β regulates t-cell Neurokinin-1 receptor internalization and function
PNAS, Mar 2010; 107: 4293 - 4298.
extracellular signal-regulated kinase 1/2 and c-jun nh2-terminal kinase, through nuclear factor-b and activator protein-1, contribute to caerulein-induced expression of substance p and Neurokinin-1 receptors in pancreatic acinar cells
J. Pharmacol. Exp. Ther., Mar 2010; 332: 940 - 948.
ketoconazole and rifampin significantly affect the pharmacokinetics, but not the safety or qtc interval, of casopitant, a Neurokinin-1 receptor antagonist
J. Clin. Pharmacol., Feb 2010; 10.1177/0091270009353761.
protein kinase c-mediated inhibition of recombinant t-type cav3.2 channels by neurokinin 1 receptors
Mol. Pharmacol., Feb 2010; 77: 202 - 210.
micrornas may mediate the down-regulation of Neurokinin-1 receptor in chronic bladder pain syndrome
Am. J. Pathol., Jan 2010; 176: 288 - 303.




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by species:
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mouse
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Antibodies:
Purified Polyclonal
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