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Human Glial growth factor (GGF2) Recombinant

NRG1

accession:  Q02297
   Size A     20ug   $ 260
   Size B      50ug  $460
   Size C      1mg  $ 4800
Domain  :  EGF
Gene  :  NRG1
Catalog no. :  RKQ022979
Source:
Optimized DNA sequence encoding Human Neuregulin-1 isoform 9 GGF2  was expressed in HEK cells.

 
Molecular weight:

Recombinant human GGF2 (Glial growth factor) is a disulfide-linked monomeric protein consisting of 230 amino acid residue subunits, and migrates as an approximately 34 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent phosphorylation of human MCF-7 cells was found to be <20ng/ml, corresponding to a specific activity of 5x 10Units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MRWRRAPRRS GRPGPRAQRP GSAARSSPPL PLLPLLLLLG TAALAPGAAA GNEAAPAGAS 

        70         80         90        100        110        120 
VCYSSPPSVG SVQELAQRAA VVIEGKVHPQ RRQQGALDRK AAAAAGEAGA WGGDREPPAA

       130        140        150        160        170        180 
GPRALGPPAE EPLLAANGTV PSWPTAPVPS AGEPGEEAPY LVKVHQVWAV KAGGLKKDSL

       190        200        210        220        230        240
LTVRLGTWGH PAFPSCGRLK EDSRYIFFME PDANSTSRAP AAFRASFPPL ETGRNLKKEV

       250        260        270        280        290        300 
SRVLCKRCAL PPRLKEMKSQ ESAAGSKLVL RCETSSEYSS LRFKWFKNGN ELNRKNKPQN

       310        320        330        340        350        360
IKIQKKPGKS ELRINKASLA DSGEYMCKVI SKLGNDSASA NITIVESNAT STSTTGTSHL

       370        380        390        400        410        420
VKCAEKEKTF CVNGGECFMV KDLSNPSRYL CKCPNEFTGD RCQNYVMASF YSTSTPFLSL   
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant GGF2 was lyophilized from a 0.2 μm filtered 0.02M PB, pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

Glial Growth Factor

Neuregulins (NDF, heregulin, GGF ARIA, or SMDF) are EGF- like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family.The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.

Related Publications:
hypertrophic neuropathies and malignant peripheral nerve sheath tumors in transgenic mice overexpressing Glial Growth Factor 3 in myelinating schwann cells
J. Neurosci., Aug 2003; 23: 7269.
in vivo analysis of schwann cell programmed cell death in the embryonic chick: regulation by axons and Glial Growth Factor
J. Neurosci., Jun 2002; 22: 4509.
Glial Growth Factor/neuregulin inhibits schwann cell myelination and induces demyelination
J. Cell Biol., Mar 2001; 152: 1289 - 1300.
the neuregulin, Glial Growth Factor 2, diminishes autoimmune demyelination and enhances remyelination in a chronic relapsing model for multiple sclerosis
PNAS, Aug 1998; 95: 10100 - 10105.
Glial Growth Factor rescues schwann cells of mechanoreceptors from denervation-induced apoptosis
J. Neurosci., Sep 1997; 17: 6697.




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