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Rabbit anti IGF-I Polyclonal

IGF-I

accession:  P01343
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA01343
Applications:
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
IGFI IHC

Immunohistochemical analysis of IGF-I expression in NZ rabbit knee joint tissue.

Cat# RPA01343 was used as primary antibody, dilution 1:200.

 
Source:
Polyclonal IGF-I antibody was produced from sera of rabbits  immunized with synthetic protein containing IGF-1  antigenic determinants. 
 
Species reactivity:
Human,Mouse,Rat
 
Purification:

Serum IgG fraction was purified by Protein A affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IGF-I

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I is synthesized as two precursor isoforms with N- and alternate C- terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF- I is identical between isoforms and is generated by proteolytic removal of the N- and C- terminal regions. Mature human IGF-I shares 94% and 96% aa sequence identity with mouse and rat IGF-I, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II. Circulating IGF-I is produced by hepatocytes, while local IGF-I is produced by many other tissues in which it has paracrine effects.

Related Publications:
mechano-transduction in osteoblastic cells involves strain-regulated estrogen receptor -mediated control of insulin-like growth factor (igf) i receptor sensitivity to ambient igf, leading to phosphatidylinositol 3-kinase/akt-dependent wnt/lrp5 receptor-independent activation of β-catenin signaling
J. Biol. Chem., Mar 2010; 285: 8743 - 8758.
IGF-I lucidity...the murky waters begin to clear?
J Appl Physiol, Mar 2010; 10.1152/japplphysiol.00252.2010.
a significant decline in IGF-I may predispose young africans to subsequent cardiometabolic vulnerability
J. Clin. Endocrinol. Metab., Mar 2010; 10.1210/jc.2009-2329.
insulin-like growth factor-1 (IGF-I) stimulated insulin receptor substrate-1 negatively regulates src homology 2 domain-containing protein-tyrosine phosphatase substrate-1 function in vascular smooth muscle cells
J. Biol. Chem., Mar 2010; 10.1074/jbc.M109.092270.
deletion of the IGF-I gene: suppressive effects on adult leydig cell development
J Androl, Mar 2010; 10.2164/jandrol.109.008680.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
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product_icon_8.jpg Mouse Insulin Like Growth Factor-1 Recombinant
product_icon_7.jpg Rabbit anti IGF-II Polyclonal
product_icon_7.jpg Rabbit anti IGF-I Polyclonal
product_icon_8.jpg Rat Insulin Like Growth Factor-I Recombinant
 
 
 
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