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Rabbit anti IFN-g Polyclonal

IFN-gamma

accession:  P01579
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA01579
Applications:
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC-F/P: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
IFN-G IHC

Immunohistochemical analysis of increased IFN-gamma expression in the mucosal surface of rat intestinal tissue.

Cat# RPA01579 was used as primary antibody, dilution 1:200.

 
Source:

Polyclonal Interferon-gamma antibody was produced from sera of rabbits  immunized with synthetic protein containing IFN-gamma  antigenic determinants. 

 

 
Species reactivity:
Mouse,Rat
 
Purification:

Serum IgG fraction was purified by Protein A affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS pH 7.4, 0.1% BSA, .
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

IFN-g

IFN-gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. IFN gamma receptors are expressed on all types of human cells with the exception of mature erythrocytes.IFN-gamma- receptor complexes are rapidly internalized by endocytosis. IFN-gamma has antiviral and antiparasitic activities and also inhibits the proliferation of a number of normal and transformed cells. Interferon-gamma synergises with TNF-alpha and TNF-beta in inhibiting the proliferation of various cell types. The growth inhibitory activities of IFN-gamma are more pronounced than those of the other interferons. However, the main biological activity of IFN-gamma appears to be immunomodulatory in contrast to the other interferons that are mainly antiviral. n T-helper cells IL2 induces the synthesis of IFN-gamma and other cytokines. IFN- gamma acts synergistically with IL1 and IL2 and appears to be required for the expression of IL2 receptors on the cell surface of T- lymphocytes. Blocking of the IL2 receptor by specific antibodies also inhibits the synthesis of IFN-gamma.

Related Publications:
diminished frequency of nk cells and cd4+IFN-g+ t cells in patients with mycotic corneal ulcers
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1945.
IFN-g-stimulated mesenchymal stromal cells acquire the ability to cross-present exogenous soluble antigens and lead to an effective immune response.
Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 4898.
regulation of tlr expression and activation in human mesenchymal stromal cells by ifn-a, IFN-g and tnf-a.
Blood (ASH Annual Meeting Abstracts), Nov 2007; 110: 1790.
positive association of tnf-857 and ifn g 874 gene polymorphisms with chronic fatigue syndrome
Ann Rheum Dis, Jun 2005; 64: 344.
measurement of functional cytomegalovirus (cmv)-specific t cells by interferon-gamma-enzyme-linked immunospot (IFN-g elispot) assay specific for a single immunodominant epitope enables the sensitive evaluation of immune recovery for cmv in patients after allogeneic stem cell transplantation (hsct) with a reduced-intensity regimen (rist).
Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 3157.




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Antibodies:
Purified Polyclonal
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