Human Fibroblast Growth Factor 21 Recombinant
Categories: CytokinesRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
Q9NSA1
Source
Optimized DNA sequence encoding Human Fibroblast Growth Factor-21 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human FGF21 is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 20kDa. Recombinant Fibroblast Growth Factor is a monomer protein consisting of 182 amino acid residue subunits,and migrates as an approximately 20kDa protein under non-reducing andreducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Protein Sequence
MDSDETGFEH SGLWVSVLAG LLLGACQAHP IPDSSPLLQF GGQVRQRYLY TDDAQQTEAH LEIREDGTVG GAADQSPESL LQLKALKPGV IQILGVKTSR FLCQRPDGAL YGSLHFDPEA CSFRELLLED GYNVYQSEAH GLPLHLPGNK SPHRDPAPRG PARFLPLPGL PPALPEPPGI LAPQPPDVGS SDPLSMVGPS QGRSPSYAS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
RecombinantFGF21 was lyophilized from.2 μm filtered PBS solution, pH7.0 .
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
