Rat Stromal Cell-Derived Factor-1 beta Recombinant
Category: Recombinant Rat Cytokines$70.00 – $2,700.00
Description
Accession
Q9QZD1
Source
Optimized DNA sequence encoding Rat Stromal Cell-Derived Factor-1 beta mature chain was expressed in Escherichia Coli.
Molecular weight
NativeRat SDF-1 beta, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately kDa. Recombinant Rat SDF-1 beta is a monomer protein consisting of73 amino acid residue subunits, migrates as an approximately kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human monocytes using a concentration range of-80 ng/ml.
Protein Sequence
MDAKVVAVLA LVLAALCISD GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKSNNRQV CIDPKLKWIQ EYLDKALNKR KM
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Rat SDF-1 beta was lyophilized from a.2 μm filtered PBS solution pH7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.