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Human BCA-1 (CXCL13) Recombinant

BCA-1

accession:  Q53X90
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  : 
Gene  :  CXCL13
Catalog no. :  RKQ53X90
Source:
Optimized DNA sequence encoding Human BCA-1 (CXCL13) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human CXCL13, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 10 kDa.

Recombinant CXCL13 is a disulfide-linked monomeric protein consisting of 88 amino acid residue subunits and migrates as an approximately 10 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human CXCR5 transfected mouse BaF3 B cells ,the ED50 is less than 20ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKFISTSLLL MLLVSSLSPV QGVLEVYYTS LRCRCVQESS VFIPRRFIDR IQILPRGNGC 

        70         80         90        100 
PRKEIIVWKK NKSIVCVDPQ AEWIQRMMEV LRKRSSSTLP VPVFKRKIP 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant BCA-1 was lyophilized from a 0.2 μm filtered 20mM PB,100mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CXCL13

The homeostatic chemokine CXCL13 (also called B cell- attracting chemokine 1 [BCA- 1] or B-lymphocyte chemoattractant [BLC]) is constitutively expressed in secondary lymphoid tissue and initiates lymphoid neogenesis when expressed aberrantly in mice. CXCL13 has also been detected in chronic inflammation associated with human lymphoid neogenesis, suggesting a pathogenic role. Follicular dendritic cells (FDCs) are generally considered to be the major source of CXCL13 both in normal and aberrant lymphoid tissue.CXCL13 has been the exclusive ligand for CXCR5, which initiates chemotaxis toward an increasing gradient in vitro and to B cell zones in lymph nodes that express CXCL13. More recently, CXCR5 expression has also been shown in DN T cells in mice , a subset of CD4 follicular Th cells, and some tonsillar CD4+CD25+ Tregs in humans

Related Publications:
serum blc/CXCL13 concentrations and renal expression of CXCL13/cxcr5 in patients with systemic lupus erythematosus and lupus nephritis
J Rheumatol, Jan 2010; 37: 45 - 52.
increased cerebrospinal fluid concentrations of the chemokine CXCL13 in active ms
Neurology, Dec 2009; 73: 2003 - 2010.
the chemokine CXCL13 in acute neuroborreliosis
J Neurol Psychiatry, Dec 2009; 10.1136/jnnp.2009.195438.
b-cell-attracting chemokine CXCL13 as a marker of disease activity and renal involvement in systemic lupus erythematosus (sle)
Nephrol. Dial. Transplant., Dec 2009; 24: 3708 - 3712.
serum levels of CXCL13 are elevated in active multiple sclerosis
Multiple Sclerosis, Nov 2009; 15: 1271 - 1279.




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Antibodies:
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product_icon_8.jpg Human BCA-1 (CXCL13) Recombinant
 
 
 
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