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Human MIP-4 (CCL18) Recombinant

MIP-4

accession:  P55774
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CCL18
Catalog no. :  RKP55774
Source:
Optimized DNA sequence encoding Human MIP-4 (CCL18) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human CCL-18, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 8kDa.

Recombinant CCL18 is a disulfide-linked homodimeric protein consisting of 70 amino acid residue subunits and migrates as an approximately 16 kDa protein under non-reducing conditions and 8kDa under reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human T cells using a concentration range of 2.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKGLAAALLV LVCTMALCSC AQVGTNKELC CLVYTSWQIP QKFIVDYSET SPQCPKPGVI 

        70         80         90 
LLTKRGRQIC ADPNKKWVQK YISDLKLNA 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/┬Ág(1EU/┬Ág).

 
Presentation:
Recombinant CCL-18 was lyophilized from a 0.2 ╬╝m filtered 20mM PB,100mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20┬░ C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2┬░ - 8┬░ C for one month, or at -20┬░ C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CCL18

CCL18, also designated dendritic cell (DC)3-derived CC chemokine 1, pulmonary and activation-regulated chemokine, alternative macrophage activation- associated CC chemokine 1, and MIP-4, is a human chemokine structurally related to CCL3.CCL18 was shown to be expressed in germinal centers of tonsils by dendritic cells and to attract mainly naive T cells, CD38ľ mantle zone B lymphocytes and DC. The production of CCL18 by APC is enhanced by Th2 cytokines like IL-4 and IL-13 and suppressed by IFN-gamma.

Related Publications:
identification of human ccr8 as a CCL18 receptor
J. Exp. Med., Sep 2013; 210: 1889 - 1898.
ab0143 angiotensin receptor type 1 and endothelin receptor type a on immune cells mediate migration and the expression of il-8 and CCL18 when stimulated by autoantibodies from systemic sclerosis patients
Ann Rheum Dis, Jun 2013; 72: A829.
249 cerebral palsy and chemokine CCL18 polymorphism in very preterm infants
Arch. Dis. Child., Oct 2012; 97: A72.
CCL18 — potential biomarker of fibroinflammatory activity in chronic periaortitis
J Rheumatol, Jul 2012; 39: 1407 - 1412.
pulmonary CCL18 recruits human regulatory t cells
J. Immunol., Jul 2012; 189: 128 - 137.




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