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Human LEC (CCL16) Recombinant

LEC

accession:  O15467
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CCL16
Catalog no. :  RKO15467
Source:
Optimized DNA sequence encoding Human LEC (CCL16) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human LEC, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 11 kDa.

Recombinant LEC/CCL16 is a disulfide-linked monomeric protein consisting of 98 amino acid residue subunits and migrates as an approximately 11 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 2.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKVSEAALSL LVLILIITSA SRSQPKVPEW VNTPSTCCLK YYEKVLPRRL VVGYRKALNC 

        70         80         90        100        110        120 
HLPAIIFVTK RNREVCTNPN DDWVQEYIKD PNLPLLPTRN LSTVKIITAK NGQPQLLNSQ 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant HCC-4 was lyophilized from a 0.2 μm filtered 20mM PB,100mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CCL16

CCL16, also known as liver- expressed chemokine or NCC-4 or lymphocyte and monocyte chemoattractant or HCC-4, is a CC chemokine that specifically attracts lymphocytes, dendritic cells, and monocytes; increases their adhesive properties; and has myelosuppressive activity. It is constitutively expressed in liver and is increased by interleukin 10 (IL-10) in activated monocytes.CCL16 is present in human plasma suggesting that it may be active outside hepatic tissue. CCR1, CCR2, CCR5, and CCR8 are the functional receptors of this chemokine.

Related Publications:
CCL16/lec powerfully triggers effector and antigen-presenting functions of macrophages and enhances t cell cytotoxicity
J. Leukoc. Biol., Jan 2004; 75: 135.
CCL16 activates an angiogenic program in vascular endothelial cells
Blood, Jan 2004; 103: 40 - 49.




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Antibodies:
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product_icon_8.jpg Human LEC (CCL16) Recombinant
 
 
 
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