Human HCC-1(CCL14) Recombinant, biotinylated
Categories: Biotinylated proteinsCCL chemokines$70.00 – $2,700.00
Description
Accession
Q16627
Source
DNA sequence encoding Human CCL-14 mature chain was expressed in Escherichia Coli. CCL14 was further labeled by Biotin for detection by Avidin conjugated secondary probes
Molecular weight
Human HCC-1, generated by the proteolytic removal of the signal peptide and propeptide and has a calculated molecular mass of approximately 9 kDa. Recombinant HCC-1 is a monomeric protein consisting of 72 amino acid residue subunits and migrates as an approximately9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Applications
Biotinylated CCL14 was designed to be used in single step detection in ELISA ,WB
Protein Sequence
MKISVAAIPF FLLITIALGT KTESSSRGPY HPSECCFTYT TYKIPRQRIM DYYETNSQCS KPGIVFITKR GHSVCTNPSD KWVQDYIKDM KEN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant HCC-1 was lyophilized from a 0.2 μm filteredmM PB,100mM NaCl solution pH.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P32246
Molecular function
