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Human MCP-4 (CCL13) Recombinant

MCP-4

accession:  Q99616
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  MCP4
Catalog no. :  RKQ99616

(*)Complete precursor sequence shown, expressed chain highlighted
 

Source:
Optimized DNA sequence encoding Human MCP-4(CCL13) mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Human MCP-4, generated by the proteolytic removal of the signal peptide
and propeptide and has a calculated molecular mass of approximately 9 kDa.

Recombinant MCP-4 is a disulfide-linked monomeric protein consisting of  76 amino acid residue subunits and migrates as an approximately 9 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human monocytes using a concentration range of 20.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKVSAVLLCL LLMTAAFNPQ GLAQPDALNV PSTCCFTFSS KKISLQRLKS YVITTSRCPQ 

        70         80         90        100 
KAVIFRTKLG KEICADPKEK WVQNYMKHLG RKAHTLKT 
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant MCP-4 was lyophilized from a 0.2 μm filtered 20mM PB solution pH 7.5, 100mM NaCl.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

MCP-4

Monocyte Chemoattractant Proteins 4 (MCP-4/CCL13) is member of a distinct, structurally-related subclass of CC chemokines. MCP-4/CCL13 is a major chemoattractants for eosinophils, basophils monocytes and T lymphocytes. The MCP protein family bind to specific G-protein- coupled receptors, initiating a signal cascade within the cell. Expression of MCP-4/CCL13 mRNA and protein is greater in the sputum, epithelium, submucosal inflammatory cells and bronchoalveolar lavage fluid of asthmatics than in healthy individuals . Involvement of MCPs has also been demonstrated in renal inflammation and atopic dermatitis and the expression of this protein is also correlated with enhanced inflammatory immune responses during immunotherapy

Related Publications:
raised MCP-4 levels in symptomatic carotid atherosclerosis: an inflammatory link between platelet and monocyte activation
Cardiovasc Res, Mar 2010; 10.1093/cvr/cvq044.
monocyte chemoattractant protein-4 (MCP-4)/ccl13 is highly expressed in cartilage from patients with rheumatoid arthritis
Rheumatology, Apr 2006; 45: 421 - 424.
il-4 differentially regulates eotaxin and MCP-4 in lung epithelium and circulating mononuclear cells
Am J Physiol Lung Cell Mol Physiol, Nov 2001; 281: 1288.
monocyte chemoattractant protein (mcp)-4 expression in the airways of patients with asthma . induction in epithelial cells and mononuclear cells by proinflammatory cytokines
Am. J. Respir. Crit. Care Med., Aug 2000; 162: 723 - 732.
eosinophil chemotactic chemokines (eotaxin, eotaxin-2, rantes, monocyte chemoattractant protein-3 (mcp-3), and MCP-4), and c-c chemokine receptor 3 expression in bronchial biopsies from atopic and nonatopic (intrinsic) asthmatics
J. Immunol., Dec 1999; 163: 6321 - 6329.




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