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Human Glia Maturation Factor beta Recombinant

GMF

accession:  P60983
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  Actin Binding
Gene  :  GMFB
Catalog no. :  RKP60983
Source:
Optimized DNA sequence encoding Human Glia Maturation Factor beta mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human Glia Maturation Factor, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 15kDa.

Recombinant Glia Maturation Factor is a disulfide-linked monomer protein consisting of 142 amino acid residue subunits, and migrates as an approximately 15kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
ED50 not determined.

 
Protein Sequence:
        10         20         30         40         50         60 
MSESLVVCDV AEDLVEKLRK FRFRKETNNA AIIMKIDKDK RLVVLDEELE GISPDELKDE 

        70         80         90        100        110        120 
LPERQPRFIV YSYKYQHDDG RVSYPLCFIF SSPVGCKPEQ QMMYAGSKNK LVQTAELTKV 

       130        140 
FEIRNTEDLT EEWLREKLGF FH 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant GMFB was lyophilized from a 0.2 μm filtered 20mM PB,130mM NaCl solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

Glia maturation factor beta

GMF-beta is a 17-kDa brain- specific protein that was isolated from bovine brain homogenate as a substance inducing the maturation of normal neurons as well as glial cells, and at first, it was considered to be a neurotrophic factor.The amino acid sequence of GMFB is highly conserved among many species, suggesting that it plays basic roles across many species. The expression of GMF-b is largely limited to the brain, especially the glial cells and some neurons . Schwann cells of the distal segment of the transected nerve express GMF-b, and this induction of GMF-b coincides with the temporal expression of nerve growth factor receptors in the cell.

Related Publications:
Glia maturation factor-gamma mediates lipopolysaccharide-induced inflammation via p38 mapk and nf-kappab.
Blood (ASH Annual Meeting Abstracts), Nov 2009; 114: 3598.
Glia maturation factor-gamma mediates neutrophil chemotaxis via p38 mapk and pten.
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 1269.
Glia maturation factor- is preferentially expressed in microvascular endothelial and inflammatory cells and modulates actin cytoskeleton reorganization
Circ. Res., Aug 2006; 99: 424 - 433.
induction of Glia maturation factor- in proximal tubular cells leads to vulnerability to oxidative injury through the p38 pathway and changes in antioxidant enzyme activities
J. Biol. Chem., Aug 2003; 278: 33519 - 33527.
Glia maturation factor produced by thymic epithelial cells plays a role in t cell differentiation in the thymic microenvironment
Int. Immunol., May 2003; 15: 557 - 564.




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