Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human PEDF Recombinant

PEDF

accession:  P36955
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  SERPIN
Gene  :  SERPINF1
Catalog no. :  RKP36955
Source:
Optimized DNA sequence encoding Human PEDF mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human PEDF, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 45kDa.

Recombinant PEDF is a disulfide-linked monomer protein consisting of 440 amino acid residue subunits, and migrates as an approximately 45kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was not determined.

 
Protein Sequence:
        10         20         30         40         50         60 
MQALVLLLCI GALLGHSSCQ NPASPPEEGS PDPDSTGALV EEEDPFFKVP VNKLAAAVSN 

        70         80         90        100        110        120 
FGYDLYRVRS STSPTTNVLL SPLSVATALS ALSLGAEQRT ESIIHRALYY DLISSPDIHG 

       130        140        150        160        170        180 
TYKELLDTVT APQKNLKSAS RIVFEKKLRI KSSFVAPLEK SYGTRPRVLT GNPRLDLQEI 

       190        200        210        220        230        240 
NNWVQAQMKG KLARSTKEIP DEISILLLGV AHFKGQWVTK FDSRKTSLED FYLDEERTVR 

       250        260        270        280        290        300 
VPMMSDPKAV LRYGLDSDLS CKIAQLPLTG SMSIIFFLPL KVTQNLTLIE ESLTSEFIHD 

       310        320        330        340        350        360 
IDRELKTVQA VLTVPKLKLS YEGEVTKSLQ EMKLQSLFDS PDFSKITGKP IKLTQVEHRA 

       370        380        390        400        410        420 
GFEWNEDGAG TTPSPGLQPA HLTFPLDYHL NQPFIFVLRD TDTGALLFIG KILDPRGP 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant PEDF was lyophilized from a 0.2 μm filtered 20mM PB,150mM NaCl  solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

PEDF

Pigment epithelium-derived factor (PEDF), a 50-kDa secreted glycoprotein, is widely expressed throughout the human body, with expression decreasing during human hepatocellular carcinoma and breast cancer progression.PEDF binds to a cell membrane receptor to exhibit multifunctional activity in many cell types but its signaling mechanisms are largely unknown. PEDF exerts anti-angiogenic activity by arresting VEGF- or bFGF-mediated endothelial cell migration, inhibiting capillary morphogenesis, and inducing endothelial cell apoptosis.In human dermal microvascular cells, PEDF induces FasL expression and subsequently activation of caspase-8, which initiates the downstream apoptotic cascade. In human umbilical vein endothelial cells (HUVECs), its induction of apoptosis was shown to depend on p38 MAPK activity and to involve activation of caspases-8 and-9.

Related Publications:
neuroprotectin d1 synthesis and corneal nerve regeneration after experimental surgery and treatment with PEDF plus dha
Invest. Ophthalmol. Vis. Sci., Feb 2010; 51: 804 - 810.
anti-permeability function of PEDF involves blockade of map kinase/gsk/beta-catenin signaling pathway and upar expression
Invest. Ophthalmol. Vis. Sci., Jan 2010; 10.1167/iovs.08-2878.
inhibition of jak2/stat3-mediated vegf upregulation under high glucose conditions by PEDF through a mitochondrial ros pathway in vitro
Invest. Ophthalmol. Vis. Sci., Jan 2010; 51: 64 - 71.
constitutive overexpression of pigment epithelium–derived factor inhibition of ocular melanoma growth and metastasis
Invest. Ophthalmol. Vis. Sci., Jan 2010; 51: 28 - 34.
pigment epithelium–derived factor (PEDF) attenuated capsaicin-induced neurotrophic keratouveitis
Invest. Ophthalmol. Vis. Sci., Nov 2009; 50: 5173 - 5180.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human PEDF Recombinant
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $