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Rabbit anti Human TNFa Polyclonal

TNF

accession:  P01375
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA01375
Applications:
Elisa: Use at a concentration range of  0.5-1ug/ml. The detection limit for TNFα is approximately 0.6 ng/well.
Source:
Polyclonal TNFa antibody was produced from sera of rabbits immunized with recombinant human Tumor necrosis factor-alpha.
 
Species reactivity:
Human
 
Purification:

Serum IgG fraction was purified by Protein A affinity chromatography.

 
Presentation:
0.2 μm filtered solution in PBS with 5% trehalose.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers.
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

TNFa

Tumor Necrosis Factor is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF- alpha while CD8(+) cells secrete little or no TNF- alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non- glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF- alpha is N-glycosylated. Homology with TNF-beta is approximately 30%. TNF- alpha forms dimers and trimers.

 




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
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