Elisa: Use at a concentration range of 0.5-1 ug/ml. The detection limit for FGF-acidic is approximately 0.15 ng/well.
Western blot: A suitable range of concentrations of this antibody for WB detection is 0.125-0.5 µg/ml.
Polyclonal aFGF antibody was produced from sera of rabbits immunized with recombinant human fibroblast growth factor acidic.
Serum IgG fraction was purified by Protein A affinity chromatography.
0.2 μm filtered solution in PBS with 5% trehalose.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers.
The lyophilized antibody is stable for at least 1 year from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.
Avoid repeated freeze/thaw cycles.
This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
aFGF and bFGF display 55%
homology which is limited,
however, to a few functional
domains. The structure of
aFGF, revealed by X-ray
crystallography, shows a
similar folding structure as
Because aFGF binds to the
same receptor as bFGF, aFGF
displays more or less the
same spectrum of activities.
It is, however, generally
less active than bFGF in
FGF receptors are encoded by
a gene family consisting of
at least four receptor
tyrosine kinases that
transduce signals important
in a variety of
related to cell growth and