Optimized DNA sequence encoding Human extracellular domain of human LIFR (CD118) including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant LIFR (CD118) is a monomer protein consisting of 794 amino acid residue subunits, due to glycosylation migrates as an approximately 125-135 kDa protein on SDS-PAGE.
>90%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD118 is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Recombinant CD118, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
LIF acts on its target cells
via the activation of the
LIF-receptor (LIFR) complex.
The LIFR complex requires
heterodimerisation of the
two low affinity components
LIFR and glycoprotein 130
(gp130), where combination
transforms a low-affinity
binding complex into a high-
affinity binding site.
Activation of the LIFR
complex can trigger several
signal transduction paIn
heterodimerization with the
LIFR, only the CBM of gp130
contacts LIF . The LIFR
contributes mainly with its
Ig-like domain to LIF
determining the lif-sensitive period for implantation using a lif-receptor antagonist
827 - 836.
loss of myocardial LIF receptor in experimental heart failure reduces cardiotrophin-1 cytoprotection. a role for neurohumoral agonists?
536 - 545.
an unusual cytokine:ig-domain interaction revealed in the crystal structure of leukemia inhibitory factor (lif) in complex with the LIF receptor PNAS,
12737 - 12742.
an in vitro and in vivo evidence that downregulation of leukemia inhibitory factor (lif) receptor (lif-r) decreases the metastatic potential of human rhabdomyosarcoma (rms) cells.
Blood (ASH Annual Meeting Abstracts),
evidence for heterodimeric association of leukemia inhibitory factor (lif) receptor and gp130 in the mouse uterus for lif signaling during blastocyst implantation
341 - 349.