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Rabbit anti GM-CSF Polyclonal


accession:  P04141
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA04141
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
Polyclonal GM-CSF antibody was produced from sera of rabbits  immunized with synthetic protein containing GM-CSF antigenic determinants. 
Species reactivity:

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.


This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non- glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T- cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM- CSF.

Related Publications:
GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions
J. Exp. Med., Sep 2009; 206: 2141 - 2149.
the transcription factors stat5a/b regulate GM-CSF-mediated granulopoiesis
Blood, Sep 2009; 10.1182/blood-2009-04-216390.
GM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace)
Blood, Sep 2009; 10.1182/blood-2009-04-215020.
biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation
PNAS, Sep 2009; 106: 15825 - 15830.
stress response genes are suppressed in mouse preimplantation embryos by granulocyte-macrophage colony-stimulating factor (GM-CSF)
Hum. Reprod., Sep 2009; 10.1093/humrep/dep307.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
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