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Rabbit anti GCSF Polyclonal

G-CSF

accession:  P09919
   Size A     100ug   $ 290
   Size B      200ug  $390
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA09919
Applications:
Elisa: Use at a concentration range of  0.5-1ug/ml,The detection limit for human G-CSF is 0.00245 ng/well
gcsf elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 1-2 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
Source:
Polyclonal G-CSF antibody was produced from sera of rabbits  immunized with recombinant human GCSF. 
 
Species reactivity:
Human
 
Purification:

Serum IgG fraction was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

G-CSF

G-CSF is secreted by monocytes, macrophages, and neutrophils after cell activation. It is produced also by stromal cells, fibroblasts, and endothelial cells. Epithelial carcinomas, acute myeloid leukemia cells and various tumor cell lines. The synthesis of G-CSF can be induced by bacterial endotoxins, TNF, IL1 and GM- CSF.Comparison of the primary sequence of G-CSF with those of the two other colony stimulating factors, GM-CSF and M-CSF, shows that the three factors are not related to each other. Murine and human G-CSF show a sequence homology of approximately 70 % at the DNA level and of 72 % at the protein level. The G-CSF receptor ,CD114, is expressed on all cells of the neutrophils and granulocytes lineage. It is expressed also in placenta cells, endothelial cells and various carcinoma cell lines.Human G-CSF is active in murine cells and vice versa. G-CSF stimulates the proliferation and differentiation of hematopoietic progenitor cells committed to the neutrophils and granulocytes lineage in a dose-dependent manner. G-CSF synergises with some other cytokines, including GM-CSF and IL4. GM-CSF and G-CSF are required, for example, to develop neutrophilic colonies in vitro. The concerted action of G-CSF and Epo is required to support the growth of mixed colonies of the early erythroid progenitors. A combination of IL4 with G- CSF has been shown to lead to synergistic suppression of the growth of some human leukemic cell lines.

Related Publications:
plerixafor (amd3100) and granulocyte colony-stimulating factor (G-CSF) mobilize different cd34+ cell populations based on global gene and microrna expression signatures
Blood, Sep 2009; 114: 2530 - 2541.
the role of granulocyte-colony stimulating factor (G-CSF) in the healthy brain: a characterization of G-CSF-deficient mice
J. Neurosci., Sep 2009; 29: 11572 - 11581.
nfi-a directs the fate of hematopoietic progenitors to the erythroid or granulocytic lineage and controls β-globin and G-CSF receptor expression
Blood, Aug 2009; 114: 1753 - 1763.
silencing of microrna-155 in mice during acute inflammatory response leads to derepression of c/ebp beta and down-regulation of G-CSF
Nucleic Acids Res., Jul 2009; 10.1093/nar/gkp577.
preoperative weekly cisplatin–epirubicin–paclitaxel with G-CSF support in triple-negative large operable breast cancer
Ann. Onc., Jul 2009; 20: 1185 - 1192.




by class:
Cytokines
Chemokines
Growth factors
CD markers
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by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
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product_icon_8.jpg Human G-CSF Recombinant (CHO cell)
product_icon_7.jpg Rabbit anti GCSF Polyclonal
product_icon_8.jpg Human CD114 Recombinant (GCSFR)
 
 
 
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