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Rabbit anti IL-10 Polyclonal


accession:  P22301
   Size A     100ug   $ 190
   Size B      200ug  $290
   Size C      500ug  $ 490
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA22301
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
Polyclonal Interleukin-10 antibody was produced from sera of rabbits  immunized with synthetic protein containing Interleukin-10  antigenic determinants. 
Species reactivity:

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.


This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


IL-10 is produced by mouse Th2-cells following their stimulation by lectins. The main source for B-cell derived IL10 in mice are Ly1 B-cells that express CD5 (Ly1) and CD11.In humans IL10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones after both antigen-specific and polyclonal activation, by B- cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells.IL10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin10 shows 73 % amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL2 and TNF-beta in Th1 T-helper subpopulations of T-cells but not of Th2 T-helper cells. This activity is antagonized by IL4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL12 synthesis by accessory cells. In the human system, IL10 is produced by, and down-regulates the function of Th1 and Th2 cells.

Related Publications:
neutralizing anti-il-10 antibody blocks the protective effect of tapeworm infection in a murine model of chemically induced colitis
J. Immunol., Jun 2005; 174: 7368 - 7375.
anti-il-10 therapeutic strategy using the immunomodulator as101 in protecting mice from sepsis-induced death: dependence on timing of immunomodulating intervention
J. Immunol., Jul 2002; 169: 384 - 392.
the role of interleukin (il)-10 in the persistence of leishmania major in the skin after healing and the therapeutic potential of anti–il-10 receptor antibody for sterile cure
J. Exp. Med., Nov 2001; 194: 1497.
generation of antigen-specific th2 cells from unprimed mice in vitro: effects of dexamethasone and anti-il-10 antibody
J. Immunol., Mar 1998; 160: 2700 - 2705.
endotoxin-induced lethality in neonatal mice is counteracted by interleukin-10 (il-10) and exacerbated by anti-il-10
Clin. Diagn. Lab. Immunol., Sep 1997; 4: 607 - 610.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
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