Optimized DNA sequence encoding Human extracellular domain of human CD16a including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD16a is a monomer protein consisting of 203 amino acid residue subunits, due to glycosylation migrates as an approximately 50 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Recombinant CD16a activity was measured by the ability to bind human IgG with an affinity constant of 180nM.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD16a is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Recombinant CD16a, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Human CD16 is encoded by two
genes, FcγRIIIA and
FcγRIIIB. FcγRIIIa is a
transmembranous isoform of
the receptor expressed on
the surface of natural
killer (NK) cells,
macrophages, a subset of
monocytes and of T cells in
association with dimers of
the γ-chain of FcεRI and/or
ζ-chain of T-cell receptors.
FcγRIIIb is a
-anchored isoform restricted
to neutrophils. FcγRIII
plays a significant role in
the clearance of immune
cytotoxicity of K/NK
affinity and kinetic analysis of fc receptor iiia (CD16a) binding to igg ligands
J. Biol. Chem.,
6210 - 6221.
phagocytosis stimulates mobilization and shedding of intracellular CD16a in human monocytes and macrophages: inhibition by hiv-1 infection
J. Leukoc. Biol.,
294 - 302.