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Monoclonal mouse anti human IL-1 beta

IL-1

accession:  P01584
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  :  Interleukin-1
Isotype  :  IgG1
Catalog no. :  RMA01584
Applications:
  • Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of IL-1b (10 µg/ml), a concentration of 4-8 µg/ml of this antibody is required.
  • ELISA:This antibody can be used at 1 μg/mL with the appropriate secondary reagents to detect human VEGF.
 
Source:
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human IL-1 beta. 
 
Species reactivity:
Human
 
Purification:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered solution at a concentration of 1mg/ml in PBS pH 7.4.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

IL-1 beta

Monocytes are the main source of secreted IL1. They express predominantly IL1- beta while human keratinocytes express large amounts of IL1-alpha. Murine macrophages display a transition from IL1-beta to IL1-alpha production during maturation of monocytes into inflammatory macrophages.There are two functionally almost equivalent forms of IL1, IL1-alpha and IL1-beta that are encoded by two different genes. IL1-beta is the predominant form in humans while it is IL1-alpha in mice. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The IL1-beta but not the IL1-alpha precursor must be processed before it can bind to the receptor. Both forms of IL1 bind to the same receptor and therefore also show similar if not identical biological activities. The receptor isolated from T-cells is expressed predominantly on T-cells and cells of mesenchymal origin. It binds both types of IL1 with equal affinity. This type is called also Type 1 receptor. It has been designated CD121a. The Type 2 receptor has been designated CD121b. It is isolated from B-cells, granulocytes, and macrophages. It is expressed predominantly on B-cells and cells of the myelomonocytic lineage and is encoded by a separate gene.

Related Publications:
cell surface-bound il-1 is an upstream regulator of the senescence-associated il-6/il-8 cytokine network
PNAS, Sep 2009; 10.1073/pnas.0905299106.
expression of bfgf and IL-1 alpha in tears of galactosemic rats: implications for wound healing and neovascularization
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 545.
cutting edge: critical role for mesothelial cells in necrosis-induced inflammation through the recognition of il-1 released from dying cells
J. Immunol., Dec 2008; 181: 8194 - 8198.
influence of interleukin 1 (il-1), il-4, and il-6 polymorphisms on genetic susceptibility to chronic osteomyelitis
Clin. Vaccine Immunol., Dec 2008; 15: 1888 - 1890.
role of acute and prolonged IL-1 alpha & beta induction on primary culture of primary porcine culture
Invest. Ophthalmol. Vis. Sci., Apr 2008; 49: 1657.




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