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Human beta Defensin-1 Recombinant

BD-1

accession:  P60022
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  Defensin
Gene  :  DEFB1
Catalog no. :  RKP60022

 

Source:
Optimized DNA sequence encoding Human beta Defensin-1 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native human BD-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 5 kDa.

Recombinant beta Defensin-1 is a disulfide-linked monomer protein consisting of 47 amino acid residue subunits, and migrates as an approximately 5 kDa protein under non-reducing and  reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1.0 ug/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MRTSYLLLFT LCLLLSEMAS GGNFLTGLGH RSDHYNCVSS GGQCLYSACP IFTKIQGTCY 

        70 
RGKAKCCK 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Human beta Defensin-1 was lyophilized from 0.2 μm filtered 100mM NaCl, 20mM PB, pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

beta defensin-1

Defensins are a large family of peptides of which two groups exist in mammals: alpha defensins and beta defensins, which are distinguishable by the spacing and connectivity of the conserved cysteine residues within the mature peptides.It is thought that defensins function in the eradication of pathogens from the host system by inserting themselves into the bacterial membrane under the influence of membrane potential, forming channels which lead to leakage of cytoplasmic molecules and cell death. Human beta-defensin-1, which has 36 amino acids, was originally isolated from hemofiltrates of patients with advanced renal failure. The BD-1 gene is expressed mainly in the urogenital tract and, to a lesser degree, in trachea and lung, BD-1 is believed to function in the antimicrobial defense of the urogenital and respiratory tracts.

Related Publications:
decreased urinary beta-defensin-1 expression as a biomarker of response to arsenic
Toxicol. Sci., Nov 2008; 106: 74 - 82.
studies of the biological properties of human -defensin 1
J. Biol. Chem., Jan 2007; 282: 1819 - 1829.
human ß-defensin-1, a potential chromosome 8p tumor suppressor: control of transcription and induction of apoptosis in renal cell carcinoma
Cancer Res., Sep 2006; 66: 8542 - 8549.
the host defense peptide beta-defensin 1 confers protection against bordetella pertussis in newborn piglets
Infect. Immun., Apr 2006; 74: 2338 - 2352.
analysis of human beta defensin-1 cytotoxicity to prostate cancer via bystander effect
AACR Meeting Abstracts, Apr 2006; 2006: 511 - 512.




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product_icon_8.jpg Human beta Defensin-1 Recombinant
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