///Mouse Growth Hormone Recombinant

Mouse Growth Hormone Recombinant

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$70.00$2,400.00

SKU: RKP06880 Tags: , , , ,

Description

Accession
P06880
Source
Optimized DNA sequence encoding Mouse Growth Hormone mature chain was expressed in Escherichia Coli.
Molecular weight
Native Mouse Growth Hormone is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. Recombinant GH is a monomer protein consisting of 191 amino acid residue subunits, and migrates as an approximately kDa protein under non-reducingand reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependentcell proliferation assay using the rat lymphoma, Nb2-11, and was found to be in the range of0.1 ng/ml.

Protein Sequence
MATDSRTSWL LTVSLLCLLW PQEASAFPAM PLSSLFSNAV LRAQHLHQLA ADTYKEFERA YIPEGQRYSI QNAQAAFCFS ETIPAPTGKE EAQQRTDMEL LRFSLLLIQS WLGPVQFLSR IFTNSLMFGT SDRVYEKLKD LEEGIQALMQ ELEDGSPRVG QILKQTYDKF DANMRSDDAL LKNYGLLSCF KKDLHKAETY LRVMKCRRFV ESSCAF
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse Growth Hormone was lyophilized from.2 μm filtered100mM NaCl,mM PB, pH.4.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
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