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Human Resistin Recombinant

Resistin

accession:  Q9HD89
   Size A     5ug   $ 70
   Size B      25ug  $160
   Size C      1mg  $ 4700
Domain  :  Resistin
Gene  :  RETN
Catalog no. :  RKQ9HD89

Source:
Optimized DNA sequence encoding Human Resistin mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human Resistin, generated by the proteolytic removal of the signal peptide
and propeptide, the molecule has a calculated molecular mass of approximately 10 kDa.

Recombinant Resistin is a disulfide-linked homodimeric protein consisting of two 93 amino acid residue subunits, and migrates as an approximately 20 kDa protein under non-reducing conditions and as a 10 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to stimulate lipolysis in cultured human adipocytes.

 
Protein Sequence:
        10         20         30         40         50         60 
MKALCLLLLP VLGLLVSSKT LCSMEEAINE RIQEVAGSLI FRAISSIGLE CQSVTSRGDL 

        70         80         90        100        110 
ATCPRGFAVT GCTCGSACGS WDVRAETTCH CQCAGMDWTG ARCCRVQP 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant human Resistin was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

Resistin

Resistin was identified by screening for genes that are induced during the differentiation of adipocytes but down- regulated in mature adipocytes. Resistin gene expression is induced during the differentiation of adipocytes. Resistin circulates in mouse serum, and its level is increased markedly in obesity. Adipocytes secrete resistin as a unique signaling molecule that may be the hormone potentially linking obesity to type II diabetes, which is characterized by target- tissue resistance to insulin. TNF-alpha is a negative regulator of Resistin gene expression and inhibits Resistin mRNA expression and protein secretion by 70-90 % in 3T3-L1 adipocytes.

Related Publications:
Resistin regulates pituitary somatotrope cell function through the activation of multiple signaling pathways
Endocrinology, Oct 2009; 150: 4643 - 4652.
Resistin: a newly identified chemokine for human cd4-positive lymphocytes
Cardiovasc Res, Sep 2009; 10.1093/cvr/cvp278.
Resistin-like molecule-β inhibits sglt-1 activity and enhances glut2-dependent jejunal glucose transport
Diabetes, Sep 2009; 58: 2032 - 2038.
Resistin is more abundant in liver than adipose tissue and is not up-regulated by lipopolysaccharide
J. Clin. Endocrinol. Metab., Aug 2009; 94: 3051 - 3057.
endoplasmic reticulum stress regulates adipocyte Resistin expression
Diabetes, Aug 2009; 58: 1879 - 1886.




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