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Human TGF alpha Recombinant

TGF alpha

accession:  P01135
   Size A     20ug   $ 70
   Size B      100ug  $160
   Size C      1mg  $ 1000
Domain  :  cystine knot growth factor
Gene  :  TGFB1
Catalog no. :  RKP01135

Source:
Optimized DNA sequence encoding Human Transforming Growth Factor Alpha mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human alpha, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 26 kDa.

Recombinant TGF-alpha is a disulfied linked  protein consisting of 50 amino acid residue subunits. TGF-Alpha migrates as an approximately  5.5 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC.

 
Protein Sequence:
 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Biological Activity:
The activity was determined by dose-dependent stimulation of thymidine uptake by BALB/c 3T3 cells, the ED50 was calculated at ≤ 0.2 ng/ml, corresponding to a specific activity of ≥ 5 x 10^6 units/mg.

 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant TGF-A is lyophilized from  0.2 μm filtered solution with no additives.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:

Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months.


Avoid repeated freeze/thaw cycles.


 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

TGF-alpha



Related Publications:
cell cycle arrest by tgf-1 enhances rsv replication in lung epithelial cells
J. Virol., Sep 2009; 10.1128/JVI.00806-09.
cutting edge: tgf-β1 and il-15 induce foxp3+ regulatory t cells in the presence of antigen stimulation
J. Immunol., Sep 2009; 183: 3574 - 3577.
nuclear factor i-c links pdgf and tgf-1 signaling to skin wound healing progression
Mol. Cell. Biol., Sep 2009; 10.1128/MCB.01921-08.
mesenchymal stem cells produce wnt isoforms and tgf1 that mediate proliferation and pro-collagen expression by lung fibroblasts
Am J Physiol Lung Cell Mol Physiol, Sep 2009; 10.1152/ajplung.90347.2008.
mechanical forces and tgfβ1 reduce podocyte adhesion through 3β1 integrin downregulation
Nephrol. Dial. Transplant., Sep 2009; 24: 2645 - 2655.




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