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Human Thymic stromal lymphopoietin Recombinant

TSLP

accession:  Q969D9
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  : 
Gene  :  TSLP
Catalog no. :  RKQ969D9
Source:
Optimized DNA sequence encoding Human Interleukin-6 mature chain was expressed in Escherichia Coli

 
Molecular weight:

Native human TSLP is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 15 kDa.
 

Recombinant TSLP is a disulfide-linked monomeric protein consisting of 132 amino acid residue subunits, and migrates as an approximately 15 kDa protein under non-reducing conditions and  reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of human IL-7Rα and human TSLP R co-transfected mouse BaF3 cells, was found to be < 3.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MFPFALLYVL SVSFRKIFIL QLVGLVLTYD FTNCDFEKIK AAYLSTISKD LITYMSGTKS 

        70         80         90        100        110        120 
TEFNNTVSCS NRPHCLTEIQ SLTFNPTAGC ASLAKEMFAM KTKAALAIWC PGYSETQINA 

       130        140        150        160 
TQAMKKRRKR KVTTNKCLEQ VSQLQGLWRR FNRPLLKQQ 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Human TSLP was Lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

TSLP



Related Publications:
TSLP and il-7 use two different mechanisms to regulate human cd4+ t cell homeostasis
J. Exp. Med., Sep 2009; 206: 2111 - 2119.
TSLP production by epithelial cells exposed to immunodeficiency virus triggers dc-mediated mucosal infection of cd4+ t cells
PNAS, Sep 2009; 106: 16776 - 16781.
helminth products bypass the need for TSLP in th2 immune responses by directly modulating dendritic cell function
PNAS, Aug 2009; 106: 13968 - 13973.
TSLP regulates intestinal immunity and inflammation in mouse models of helminth infection and colitis
J. Exp. Med., Mar 2009; 206: 655 - 667.




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