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Recombinant Human Phosphoinositide 3-kinase p110d/p85a

rHuPI3KdGST

accession: 
   Size A       $ 70
   Size B       $160
   Size C       $
Domain  : 
Gene  : 
Catalog no. :  SKU1571
Source:
Optimized DNA sequence encoding Human Interleukin-6 mature chain was expressed in Escherichia Coli

 
Molecular weight:
Recombinant human IL-6, generated by the proteolytic removal of the signal peptide
and propeptide, is a disulfide-linked homodimeric protein consisting of two 114 amino acid residue subunits. The molecule has a calculated molecular mass of approximately 26 kDa. rhIL-6 migrates as an approximately 32 kDa protein under non-reducing
conditions and as a 15 - 16 kDa protein under reducing conditions in SDS-PAGE.

 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.

 
Protein Sequence:
  
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Interleukin-6 was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 



 




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Antibodies:
Purified Polyclonal
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product_icon_3.jpg Recombinant Human Phosphoinositide 3-kinase p110d/p85a
 
 
 
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