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Human Thrombopoietin Recombinant

TPO

accession:  P40225
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3900
Domain  :  four helix cytokine core
Gene  :  THPO
Catalog no. :  RKP40225
Source:
Optimized DNA sequence encoding Human TPO erythropoeitin domain was expressed in Chinese Hamster Ovary cells.

 
Molecular weight:

Native Human TPO, generated by the proteolytic removal of the signal peptide
and propeptide,and has a calculated molecular mass of approximately 35 kDa.

Recombinant TPO is disulfide-linked monomer protein consisting of  332 amino acid residue subunits, and migrates due to glycosylation as an approximately 80 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent proliferation of Mo7e cells, was found to be in the range of 1.0-2.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MELTELLLVV MLLLTARLTL SSPAPPACDL RVLSKLLRDS HVLHSRLSQC PEVHPLPTPV 

        70         80         90        100        110        120 
LLPAVDFSLG EWKTQMEETK AQDILGAVTL LLEGVMAARG QLGPTCLSSL LGQLSGQVRL 

       130        140        150        160        170        180 
LLGALQSLLG TQLPPQGRTT AHKDPNAIFL SFQHLLRGKV RFLMLVGGST LCVRRAPPTT 

       190        200        210        220        230        240 
AVPSRTSLVL TLNELPNRTS GLLETNFTAS ARTTGSGLLK WQQGFRAKIP GLLNQTSRSL 

       250        260        270        280        290        300 
DQIPGYLNRI HELLNGTRGL FPGPSRRTLG APDISSGTSD TGSLPPNLQP GYSPSPTHPP 

       310        320        330        340        350 
TGQYTLFPLP PTLPTPVVQL HPLLPDPSAP TPTPTSPLLN TSYTHSQNLS QEG 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Thrombopoietin was lyophilized from a 0.2 μm filtered solution in PBS, pH 7.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Thrombopoietin

TPO, known also as TSF (thrombopoiesis stimulating factor), for mediator substances that function as natural stimulators of megakaryocytopoiesis and thus, promote the differentiation of platelets. TPO stimulates an increase in the sizes and numbers of megakaryocytes. It also increases the number of small acetyl-cholinesterase positive cells that are early precursor cells of the megakaryocytic lineage. TPO is a protein of 332 amino acids that shows significant homology with Epo (erythropoietin) in its N-terminal domain. A comparison of human and porcine TPO sequences shows 83 % identity between the erythropoietin-like domains but only 67 % between the C- terminal domains.

Related Publications:
effect of the non-peptide Thrombopoietin receptor agonist eltrombopag on bone marrow cells from patients with acute myeloid leukemia and myelodysplastic syndrome
Blood, Aug 2009; 10.1182/blood-2009-04-219493.
association of hereditary thrombocythemia and distal limb defects with a Thrombopoietin gene mutation
Blood, Aug 2009; 114: 1655 - 1657.
Thrombopoietin controls proliferation of embryonic multipotent hematopoietic progenitors
Genes Cells, Jul 2009; 14: 851 - 860.
second generation Thrombopoietin agents for treatment of chronic idiopathic thrombocytopenic purpura in adults
Journal of Oncology Pharmacy Practice, Jun 2009; 10.1177/1078155209337668.
ligand-independent Thrombopoietin mutant receptor requires cell surface localization for endogenous activity
J. Biol. Chem., May 2009; 284: 11781 - 11791.




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