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Human Fibroblast Growth Factor-9 Recombinant

FGF-9

accession:  P31371
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 3500
Domain  :  HBGF/IL1
Gene  :  FGF9
Catalog no. :  RKP31371
Source:
Optimized DNA sequence encoding Human FGF9 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native human FGF-9 is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 23 kDa.

Recombinant Fibroblast Growth Factor-9 is a disulfide-linked monomer protein consisting of 208 amino acid residue subunits, and migrates as an approximately 23 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent  proliferation of  mouse 3T3 cells expressing FGF receptors and was found to be in the range of 5 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MAPLGEVGNY FGVQDAVPFG NVPVLPVDSP VLLSDHLGQS EAGGLPRGPA VTDLDHLKGI 

        70         80         90        100        110        120 
LRRRQLYCRT GFHLEIFPNG TIQGTRKDHS RFGILEFISI AVGLVSIRGV DSGLYLGMNE 

       130        140        150        160        170        180 
KGELYGSEKL TQECVFREQF EENWYNTYSS NLYKHVDTGR RYYVALNKDG TPREGTRTKR 

       190        200        210 
HQKFTHFLPR PVDPDKVPEL YKDILSQS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant FGF-9 was lyophilized from a 0.2 μm filtered PBS solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

FGF-9

FGF-9 is an autocrine and paracrine prostatic growth factor expressed by prostatic stromal cells. FGF-9 induces osteoblast proliferation and new bone formation in a bone organ assay. FGF-9 is produced by many prostate cancer cells and contributes to prostate cancer-induced new bone formation and may participate in the osteoblastic progression of prostate cancer in bone. It is also a an autocrine and/or paracrine neurotrophic factor that promotes the survival of motoneurons and upregulates choline acetyl-transferase activity.FGF-9 enhances survival of AChE-positive neurons, increases their mean soma size. It also up- regulates their choline acetyltransferase activity as potently as NGF and the effect is greater than that elicited by bFGF, CNTF, or GDNF. FGF-9 acts as a survival factor for neurons but does not promote neurite outgrowth. FGF-9 has been shown to mediate its effects by binding to FGF receptors. It efficiently activates the FGFR2c splice form of FGFR2 and the FGFR3b and FGFR3c splice isoforms of FGFR3.

Related Publications:
in vitro effect of cntf, fgf–9, and il–1 on human optic nerve head astrocytes.
Invest. Ophthalmol. Vis. Sci., May 2004; 45: 2181.




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