///Mouse Interleukin-1 beta Recombinant

Mouse Interleukin-1 beta Recombinant

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$70.00$4,700.00

SKU: RKP10749 Tags: , , ,

Description

Accession
P10749
Source
Optimized DNA sequence encoding Mouse Interleukin-1 beta mature chain was expressed in Escherichia Coli.
Molecular weight
Mature Mouse IL-1 beta, is generated by the proteolytic removal of the signal peptide and propeptide.The molecule has a calculated molecular mass of approximately 17 kDa. Recombinant mouse IL-1 beta is a monomer protein consisting of 153 amino acid residue subunits. Recombinant mouse IL-1 beta migrates as an approximately 17 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by thedose-dependent stimulation of thymidine uptake by murine D10S cells is ≤.0 pg/ml, corresponding to a specific activity of ≥5 x units/mg.
Protein Sequence
MATVPELNCE MPPFDSDEND LFFEVDGPQK MKGCFQTFDL GCPDESIQLQ ISQQHINKSF RQAVSLIVAV EKLWQLPVSF PWTFQDEDMS TFFSFIFEEE PILCDSWDDD DNLLVCDVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF LGNNSGQDII DFTMESVSS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Mouse IL-1 beta was lyophilized from a.2 μm filtered solution in PBS, pH.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
Molecular function
Molecular function

Methods

Selection of a β-TC3 cell population resistant to cytokine-mediated cytotoxicity.

  • Cytotoxicity assay in β-TC3 (left) and β-TC3R (right) after cytokine treatment (100 IU/ml IL-1β, 100 IU/ml IFN-γ or their combination, for 72 hours).

Preliminary investigation of CYP27B1 regulation in hGF and hPDLC.

  • IL-1β and sodium butyrate significantly up-regulated CYP27B1 mRNA expression independently of 1,25OH2D3.

IL-1 increases primary cilia length in chondrocytes.

  • b Frequency histogram of chondrocyte cilia length showing elongation of cilia associated with IL-1β treatment (10 ng mL−1, 24 h).

MicroRNA-124 and CCAAT-enhancer-binding protein (C/EBP)-α expression after intraplantar interleukin (IL)-1β in wild-type (WT) and LysM-G protein–coupled receptor kinase (GRK)2mice.

  • Control WT and LysM-GRK2+/− mice received an intraplantar injection of 1 ng IL-1β.

ELISA

  • ELISA assays for TNF, sICAM-1, and Total MMP-9 , and MCP-1, IL-1β, IL-6, and IL-12 p40 were performed according to the manufacturers’ protocols.
  • All measurements were performed in triplicates.

Induction of IL-6 and IL-1β in response to demand is impaired in Eif2b5R132H/R132H mice brain.

  • Total RNA was extracted and subjected to qRT-PCR analysis of IL-6 and IL-1β mRNA levels.

CD4+ T cell cultures

  • CD4+ T cells were purified using DynaBeads FlowComp mouse CD4 kit and were activated on αCD3/αCD28 coated plates under the following culture conditions: TH1 conditions: αIL4 10μg/ml (11B11) 0.1μg/ml IFNγ , 10u/ml IL12, 40u/ml; TH2 conditions: αIL12 10μg/ml (Tosh, ), αIFNγ 10μg/ml (XMG1.2, ), 10ng/ml IL4 , 40u/ml; TH17 conditiions: IL-6 25ng/ml , TGFβ 2ng/ml , IL1β 10ng/ml , αIL4 10 μg/ml (11B11, ), αIFNγ 10μg/ml (XMG1.2, ), and αIL12 10μg/ml (Tosh, ).
  • IL2 (40u/ml) was added for the TH17 cultures in

IpaH0722 selectively inhibits PMA–induced activation of the NF-κB pathway.

  • After 24 h, cells were treated with TNF-α, PMA, LPS, or IL-1β for 3 h and luciferase activity was measured.

Effects of anti‐inflammatory drugs and iNOS‐selective inhibitor on iNOS‐derived NO production in ex vivo aortic segments.

  • NO production in response to 10 ng/mL of TGF‐β1, 500 μmol/L of dexamethasone, 500 μmol/L of aspirin, or 500 μmol/L of aminoguanidine with or without the stimulation of 10 ng/mL IL‐1β in S3KO and WT endothelium‐denuded aortic segments.

Isolation and Culture of Primary Keratinocytes

  • For keratinocyte G1/S phase transition assays, cells were starved for 3 h as above and then stimulated by the addition EMEM supplemented with 0.2 µM TPA, 10 ng/ml of receptors for transmembrane tyrosine kinases , 10 ng/ml IL1β , 100 ng/ml IL6, or 8% calcium-chelated fetal calf serum.
  • After 4 h, cells were incubated with the EdU reactive for 30 min, and the percentage of cells in S phase (EdU+) determined using the Click-iT EdU Alexa Fluor 647 Flow Cytometry Assay Kit .
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