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Toxoplasma Gondii Rhoptry protein Recombinant

TOXGO ROP4

accession:  Q9U0D6
   Size A       $
   Size B      0.5mg  $800
   Size C      1mg  $ 1100
Domain  : 
Gene  :  ROP4
Catalog no. :  RKQ9U0D6
Source:
DNA sequence encoding immunodominant  fragment of Toxoplasma ROP4 protein was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant TOXGO ROP4 encodes the immunodominant sequences of the Toxoplasma Dense Granule protein.

Recombinant ROP4 contains an N terminal GST purification tag and migrates as an approximately 43 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE.
 
Applications:

Toxoplasma ROP4 antigen is suitable for ELISA and Western blots, excellent antigen for detection of Toxoplasma with minimal specificity problems.

 

 Specificity:
Immunoreactive with sera of Toxopalsma-infected individuals.
 
Presentation:
Lyophilized from 50mM Tris-HCl, 60mM NaCl and 50% glycerol.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This  product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

TOXGO ROP4

ROP4, a previously uncharacterized member of the ROP2 family of proteins. ROP4 is secreted from the rhoptries during or shortly after invasion and associates with the PVM, presumably through its predicted transmembrane domain. ROP4 becomes phosphorylated in the infected cell, by either host cell protein kinase(s) or parasite kinase(s) activated by host cell factors.

 




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Antibodies:
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