Rat Interleukin 10 Recombinant
Categories: Interferon-IL10 familyRecombinant Rat Cytokines$70.00 – $4,700.00
Description
Accession
P29456
Source
Optimized DNA sequence encoding rat Interleukin-10 mature chain was expressed in Escherichia Coli.
Molecular weight
NativeRat Interleukin-10 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately kDa. RecombinantRat Interleukin-10is a monomer protein consisting of161 amino acid residue subunits, and migrates as an approximately19 kDa protein under non-reducing andreducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the dose-dependentinhibition of antigen-specific T cell proliferation is less than ng/ml.
Protein Sequence
MPGSALLCCL LLLAGVKTSK GHSIRGDNNC THFPVSQTHM LRELRAAFSQ VKTFFQKKDQ LDNILLTDSL LQDFKGYLGC QALSEMIKFY LVEVMPQAEN HGPEIKEHLN SLGEKLKTLW IQLRRCHRFL PCENKSKAVE QVKNDFNKLQ DKGVYKAMNE FDIFINCIEA YVTLKMKN
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant rat Interleukin-10 was lyophilized from a 0.2 μm filtered PBS solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
IL-10 and IL-12 secretion as assessed by ELISA.
- IL-10 secreted in monocultures of MSCs, iDCs and mDCs and in the co-culture of MSC-DC (MSC:DC = 1∶10).
