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Human MIG Recombinant

MIG

accession:  Q07325
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CXCL9
Catalog no. :  RKQ07325
Source:
Optimized DNA sequence encoding Human MIG mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human MIG(CXCL9) is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 11 kDa.

Recombinant MIG(CXCL9) is a disulfide-linked monomeric protein consisting of  104 amino acid residue subunits, migrates as an approximately 11kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human peripheral blood T lymphocytes using a concentration range of 10.0-80.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MKKSGVLFLL GIILLVLIGV QGTPVVRKGR CSCISTNQGT IHLQSLKDLK QFAPSPSCEK 

        70         80         90        100        110        120 
IEIIATLKNG VQTCLNPDSA DVKELIKKWE KQVSQKKKQK NGKKHQKKKV LKVRKSQRSR 


QKKTT 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant MIG/CXCL9 was lyophilized from 1.0 mg/ml solution in 25mM NaCl 10mM PB pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

CXCL9

MIG(CXCL9) protein belongs to the family of chemotactic cytokines known as Chemokines. The synthesis of MIG(CXCL9) is specifically induced in macrophages and in other cells by IFN-gamma.Human neutrophils produce mig in response to IFN-gamma in combination with either TNF- alpha or bacterial lipopolysaccharides and this response is blocked by IL-10 and IL-4.mig is a chemoattractant for stimulated but not resting T-cells,however it is not active on neutrophils or monocytes.

Related Publications:
sufa of the opportunistic pathogen finegoldia magna modulates actions of the antibacterial chemokine mig/CXCL9, promoting bacterial survival during epithelial inflammation
J. Biol. Chem., Jul 2009; 10.1074/jbc.M109.025957.
monokine induced by interferon- (mig/CXCL9) is derived from both donor and recipient sources during rejection of class ii major histocompatibility complex disparate skin allografts
Am. J. Pathol., Jun 2009; 174: 2172 - 2181.
monokine induced by interferon (ifn) (CXCL9) and ifn inducible t-cell -chemoattractant (cxcl11) involvement in graves’ disease and ophthalmopathy: modulation by peroxisome proliferator-activated receptor- agonists
J. Clin. Endocrinol. Metab., May 2009; 94: 1803 - 1809.
role of t cell chemoattractants cxcl10/ip10 and CXCL9/mig in high risk corneal allograft transplantation
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1977.
regulation of CXCL9/10/11 in oral keratinocytes and fibroblasts
Journal of Dental Research, Dec 2008; 87: 1160 - 1165.




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