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Human Bone Morphogenetic protein-4 Recombinant

BMP-4

accession:  P12644
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3550
Domain  :  TGFB
Gene  :  bmp4
Catalog no. :  RKP12644
Source:
Optimized DNA sequence encoding Human Bone Morphogenetic protein-4 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native human Bone Morphogenetic protein-4 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 13 kDa.

Recombinant BMP-4 is a disulfide-linked monomeric protein consisting of 117 amino acid residue subunits, and migrates as an approximately as a 13 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by its ability to induce alkaline phosphatase production in mouse ATDC5 chondrogenic and was found to be in the range of 0.1-0.2 ug/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MIPGNRMLMV VLLCQVLLGG ASHASLIPET GKKKVAEIQG HAGGRRSGQS HELLRDFEAT 

        70         80         90        100        110        120 
LLQMFGLRRR PQPSKSAVIP DYMRDLYRLQ SGEEEEEQIH STGLEYPERP ASRANTVRSF 

       130        140        150        160        170        180 
HHEEHLENIP GTSENSAFRF LFNLSSIPEN EVISSAELRL FREQVDQGPD WERGFHRINI 

       190        200        210        220        230        240 
YEVMKPPAEV VPGHLITRLL DTRLVHHNVT RWETFDVSPA VLRWTREKQP NYGLAIEVTH 

       250        260        270        280        290        300 
LHQTRTHQGQ HVRISRSLPQ GSGNWAQLRP LLVTFGHDGR GHALTRRRRA KRSPKHHSQR 

       310        320        330        340        350        360 
ARKKNKNCRR HSLYVDFSDV GWNDWIVAPP GYQAFYCHGD CPFPLADHLN STNHAIVQTL 

       370        380        390        400        410 
VNSVNSSIPK ACCVPTELSA ISMLYLDEYD KVVLKNYQEM VVEGCGCR 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant BMP-4 was lyophilized from a 0.2 μm filtered 20mM Na2CO3, pH 9.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

BMP-4

BMPs are proteins that act to induce the differentiation of mesenchymal-type cells into chondrocytes and osteoblasts before initiating bone formation. They promote the differentiation of cartilage-forming cells and bone-forming cells near sites of fractures but also at ectopic locations. Some of the proteins induce the synthesis of alkaline phosphatase and collagen in osteoblasts. Some BMPs act directly on osteoblasts and promote their maturation while at the same time suppressing myogenous differentiation. Other BMPs promote the conversion of typical fibroblasts into chondrocytes and are capable also of inducing the expression of an osteoblast phenotype in non-osteogenic cell types. Intracellular signaling following engagement of receptors for some BMP proteins has been shown to involve the action of SMAD proteins. BMP4 and BMP7 have been shown to be involved also in the differentiation of sympathetic neurons. They enhance the formation of adrenergic sympathetic neurons in cultures of neural crest cells.

Related Publications:
smad, pi3k/akt, and wnt-dependent signaling pathways are involved in BMP-4-induced esc self-renewal
Stem Cells, Aug 2009; 27: 1858 - 1868.
molecular interaction of BMP-4, tgf-β, and estrogens in lactotrophs: impact on the prl promoter
Mol. Endocrinol., Jul 2009; 23: 1102 - 1114.
suppression of vegf expression and lesion size in laser-induced cnv in a mouse model overexpressing BMP-4
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 1193.
altered shear stress stimulates upregulation of endothelial vcam-1 and icam-1 in a BMP-4– and tgf-β1–dependent pathway
Arterioscler Thromb Vasc Biol, Feb 2009; 29: 254 - 260.
the bone morphogenetic protein (bmp)-4 is involved in the regulation of human megakaryocytic differentiation during thrombopoietin signaling.
Blood (ASH Annual Meeting Abstracts), Nov 2008; 112: 1339.




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