Human Interleukin-8 (77AA) Recombinant
Categories: CXC chemokinesRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
P10145
Source
Optimized DNA sequence encoding Human Interleukin-8 mature chain was expressed in Escherichia Coli.
Molecular weight
Native human Interleukin-8, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately kDa. Recombinant Interleukin-8 is a monomer protein consisting of78 amino acid residue subunits, migrates as an approximately9 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Activity was determined by the ability to chemoattract humanpheripheral blood monocyteswas found to be in the range of.0-100.0 ng/ml.
Protein Sequence
MTSKLAVALL AAFLISAALC EGAVLPRSAK ELRCQCIKTY SKPFHPKFIK ELRVIESGPH CANTEIIVKL SDGRELCLDP KENWVQRVVE KFLKRAENS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Interleukin-8 was lyophilized from a 0.2 μm filtered PBS solution pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P25024
Interactor
P25025
Interactor
Q16570
Biological Process
Biological Process
Molecular function
