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Human Parathyroid Hormone Recombinant

PTH

accession:  P01270
   Size A     20ug   $ 70
   Size B      100ug  $160
   Size C      1mg  $ 800
Domain  :  PTH
Gene  :  PTH
Catalog no. :  RKP01270
Source:
Optimized DNA sequence encoding Human PTH mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Human PTH, generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 9 kDa.

Recombinant Human PTH-84 is a disulfide-linked homodimeric protein consisting of 84 amino acid residue subunits, and migrates as an approximately 9 kDa under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity was determined by the dose-dependent proliferation of Human  UMR106 cells and was found to be 1x104 IU/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MIPAKDMAKV MIVMLAICFL TKSDGKSVKK RSVSEIQLMH NLGKHLNSME RVEWLRKKLQ 

        70         80         90        100        110 
DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant PTH-84 was lyophilized from a 0.2 μm filtered PB solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

PTH

Parathyroid hormone is a circulating hormone that acts as the central regulator of calcium metabolism by directly targeting bone, kidney, and intestine. The classical concept of PTH action is that it regulates serum calcium levels by stimulating bone resorption; however, intermittent administration of PTH selectively stimulates bone formation. It is now known that PTH binds to its receptor PTH1R, and activates the G protein α subunits.Besides PKA and PKC activation, PTH also regulates MAPKs including p42/p44 ERKs, p38, and c-Jun N-terminal kinase subtypes.

Related Publications:
endogenous parathyroid hormone-related protein regulates the expression of PTH type 1 receptor and proliferation of vascular smooth muscle cells
Mol. Endocrinol., Oct 2009; 23: 1681 - 1690.
PTH-induced internalization of apical membrane napi2a: role of actin and myosin vi
Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00260.2009.
motoring down the microvilli. editorial focus on "PTH-induced internalization of apical membrane napi2a: role of actin and myosin vi"
Am J Physiol Cell Physiol, Sep 2009; 10.1152/ajpcell.00423.2009.
simultaneous control of PTH and caxp is sustained over three years of treatment with cinacalcet hcl
Clin. J. Am. Soc. Nephrol., Sep 2009; 4: 1465 - 1476.
human PTH gene regulation in vivo using transgenic mice
Am J Physiol Renal Physiol, Sep 2009; 297: F713 - F719.




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