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Human Platelet Derived Growth Factor AB Recombinant

PDGF

accession:  P01127
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3500
Domain  :  cystine knot growth factor
Gene  :  PDGFA
Catalog no. :  RKP011271
Source:
Optimized DNA sequence encoding Human PDGF-AB mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant Human PDGF-AB  is a disulfide-linked heterodimer protein consisting of 235 amino acid residue subunits (alpha+beta chains), and migrates as an approximately 26 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by BALB/c 3T3 was found to be in the range of 1 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV 

        70         80         90        100        110        120       
HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR

       130        140        150        160        17
KRLKPTSLGS LTIAEPAMIA ECKTRTEVFE ISRRLIDRTN ANFLV 

       130        140        150        160        170        180 
WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC 

       190        200        210        220        230        240 
ETVAAARPVT  

(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
PDGF-AB was lyophilized from a 0.2 μm filtered solution in PBS pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

PDGF AB

PDGF is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets. Platelets synthesize a mixture of the three possible isoforms (BB,AB,AA)while fibroblasts stimulated with EGF synthesize AA homodimers. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells, and endothelial cells.Two related receptors, are PDGFRalpha (CD140a) or PDGFRbeta ( CD140b).In contrast to many other cytokines PDGF is not released into the circulation. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix which facilitates local concentration of the factor. The factor functions as a local autocrine and paracrine growth factor. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF regulates the synthesis of its own receptor and also influences the expression of membrane receptors for IL1, EGF, 5-Hydroxytryptamine, LDL, transferrin, and muscarinergic receptor.

Related Publications:
intra- and post-dialytic platelet activation and pdgf-ab release: cellulose diacetate vs polysulfone membranes
Nephrol. Dial. Transplant., Jun 2001; 16: 1222 - 1229.
intimal hyperplasia recurs after removal of pdgf-ab and -bb inhibition in the rat carotid artery injury model
Arterioscler Thromb Vasc Biol, Nov 2000; 20: e89 - e95.
pdgf-ab release during and after haemodialysis procedure
Nephrol. Dial. Transplant., Oct 1999; 14: 2413 - 2419.
angiotensin-converting enzyme inhibition abolishes medial smooth muscle pdgf-ab biosynthesis and attenuates cell proliferation in injured carotid arteries : relationships to neointima formation
Circulation, Sep 1997; 96: 1631 - 1640.
transduction of mitogenic activity of platelet-derived growth factor (pdgf) ab by pdgf-beta receptor without participation of pdgf-alpha receptor in vascular smooth muscle cells
J. Biol. Chem., Aug 1993; 268: 17045 - 17050.




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