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Human Platelet Derived Growth Factor-AA Recombinant

PDGF

accession:  P04085
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3500
Domain  :  Cystine knot growth factor
Gene  :  PDGFA
Catalog no. :  RKP04085
Source:
Optimized DNA sequence encoding Human PDGF-AA mature chain was expressed in CHO Cells.

 
Molecular weight:

Human PDGF-AA, is generated by the proteolytic removal of the signal peptide
and propeptide. The molecule has a calculated molecular mass of approximately 28 kDa.

Recombinant PDGF-AA is a disulfide-linked homodimeric protein consisting of two 126 amino acid residue subunits, migrates due to glycosylation as an approximately 29 kDa protein under non-reducing and as 16kDa under reducing conditions in SDS-PAGE.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of thymidine uptake by Balb/c 3T3 cells is ≤ 1 ng/ml, corresponding to a specific activity of > 1 x 106 units/mg

 
Protein Sequence:
        10         20         30         40         50         60 
MRTLACLLLL GCGYLAHVLA EEAEIPREVI ERLARSQIHS IRDLQRLLEI DSVGSEDSLD 

        70         80         90        100        110        120 
TSLRAHGVHA TKHVPEKRPL PIRRKRSIEE AVPAVCKTRT VIYEIPRSQV DPTSANFLIW 

       130        140        150        160        170        180 
PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV KVAKVEYVRK KPKLKEVQVR LEEHLECACA 

       190        200        210 
TTSLNPDYRE EDTGRPRESG KKRKRKRLKP T 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant PDGF-AA was lyophilized from a 0.2 μm filtered 150mM NaCl, 50mM Tris  solution pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

PDGF-AA

PDGF is synthesized mainly by megakaryocytes. It is stored in the alpha granules of platelets from which it is released after cell activation of platelets. Platelets synthesize a mixture of the three possible isoforms (BB,AB,AA)while fibroblasts stimulated with EGF synthesize AA homodimers. PDGF receptors are expressed in fibroblasts, osteoblasts, chondroblasts, smooth muscle cells, glial cells, and endothelial cells.Two related receptors, are PDGFRalpha (CD140a) or PDGFRbeta ( CD140b).In contrast to many other cytokines PDGF is not released into the circulation. PDGF binds to several plasma proteins and also to proteins of the extracellular matrix which facilitates local concentration of the factor. The factor functions as a local autocrine and paracrine growth factor. In the adult organism PDGF is involved in wound healing processes. The aberrant expression of PDGF is observed with vascular proliferative diseases such as atherosclerosis. PDGF regulates the synthesis of its own receptor and also influences the expression of membrane receptors for IL1, EGF, 5-Hydroxytryptamine, LDL, transferrin, and muscarinergic receptor.

Related Publications:
pdgf receptor-β modulates metanephric mesenchyme chemotaxis induced by pdgf aa
Am J Physiol Renal Physiol, Feb 2009; 296: F406 - F417.
persistent cytomegalovirus infection is associated with increased expression of tgf-ß1, PDGF-AA and icam-1 and arterial intimal thickening in kidney allografts
Nephrol. Dial. Transplant., Apr 2005; 20: 790 - 796.
il-13 and il-1β promote lung fibroblast growth through coordinated up-regulation of PDGF-AA and pdgf-rα
FASEB J, May 2004; 10.1096/fj.03-1492fje.
pdgf (alpha)-receptor is unresponsive to PDGF-AA in aortic smooth muscle cells from the ng2 knockout mouse
J. Cell Sci., Mar 1999; 112: 905 - 915.
pdgf aa as mediator in nicotine-dependent carcinogenesis
Carcinogenesis, Sep 1996; 17: 1813 - 1818.




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Antibodies:
Purified Polyclonal
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