/, PDGF family, Recombinant Human Cytokines/Human Platelet Derived Growth Factor-AA Recombinant

Human Platelet Derived Growth Factor-AA Recombinant

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$70.00$3,500.00

SKU: RKP04085 Tags: , , , ,

Description

Accession
P04085
Source
Optimized DNA sequence encoding Human PDGF-AA mature chain was expressed in CHO Cells.
Molecular weight
Human PDGF-AA, is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately kDa. Recombinant PDGF-AA is a disulfide-linked homodimeric protein consisting of two 125 amino acid residue subunits, migrates due to glycosylation as an approximately 29 kDa protein under non-reducing and as 14 kDa under reducing conditions in SDS-PAGE.
Purity
>98%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determinedby the dose-dependent stimulation of thymidine uptake by Balb/cT3 cells is ≤ ng/ml, corresponding to a specific activity of > x units/mg
Protein Sequence
MRTLACLLLL GCGYLAHVLA EEAEIPREVI ERLARSQIHS IRDLQRLLEI DSVGSEDSLD TSLRAHGVHA TKHVPEKRPL PIRRKRSIEE AVPAVCKTRT VIYEIPRSQV DPTSANFLIW PPCVEVKRCT GCCNTSSVKC QPSRVHHRSV KVAKVEYVRK KPKLKEVQVR LEEHLECACA TTSLNPDYRE EDTGRPRESG KKRKRKRLKP T
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Presentation
Recombinant PDGF-AAwas lyophilized from a.2 μm filteredmM NaCl,mM Tris solution pH7.0.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Interactor
Interactor
Molecular function
Molecular function
Molecular function

Methods

Differentiation of spinal cord NSPC

  • NSPC were assessed for multipotentiality by plating onto matrigel in SFM with 1% fetal bovine serum (FBS) in the absence of growth factors for 4 weeks to allow the cells to differentiate.
  • To assess the effect of exogenous factors on NSPC differentiation, NSPC were plated in EGF/FGF2 medium at a density of 105 cells/well into 24-well culture plates coated with matrigel.
  • Cultures were incubated for 1 week, and then the medium was replaced with one of the following factors in SFM: 40 and 100 ng/ml PDGF-AA to promote oligodendrocyte differentiation, and 1 and 4 mM dbcAMP to promote neuronal differentiation.
  • Controls included 1% FBS and SFM.
  • Cultures were incubated at 37°C for an additional 4 weeks and the media changed every week.
  • The phenotypic expression pattern of NSPC in differentiating culture conditions was examined by immunostaining as described below.
  • The total number of cells counted ranged from 180 – 400 cells…
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