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Epstein-Barr Virus EBNA1 Recombinant

EBV EBNA1

accession:  P03211
   Size A       $
   Size B      0.5mg  $800
   Size C      1mg  $ 1100
Domain  : 
Gene  :  EBNA1
Catalog no. :  RKP03211
Source:
DNA sequence encoding immunodominant  fragment of Epstein-Barr nuclear antigen 1 expressed in Escherichia Coli.

 
Molecular weight:

Recombinant EBV EBNA-1 encodes the immunodominant sequences of theEpstein-Barr Virus nuclear antigen 1 protein. 

Recombinant EBV EBNA1 contains an N terminal GST purification tag and migrates as an approximately 46 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE.
 
Applications:

Recombinant human EBV EBNA1 antigen is suitable for ELISA and Western blots, excellent antigen for detection of EBV with minimal specificity problems.

 

 Specificity:
Immunoreactive with sera of EBV infected individuals.
 
Presentation:
Lyophilized from 50mM Tris-HCl pH 8, 10mM glutation, 60mM NaCl and 0.5% sarcosyl.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This  product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

EBV EBNA1

EBNA1 is the only EBV protein consistently expressed in all proliferating infected cells and plays several important roles in EBV latent infection, including the initiation of EBV DNA replication, the mitotic segregation of the EBV genomes, and transcriptional activation of other EBV latency proteins. several pieces of evidence suggest that EBNA1 plays a direct role in cellular transformation by EBV. First, EBNA1 is expressed in all EBV-associated tumors and is the only viral protein expressed in some of these tumors. Second, transgenic mice expressing EBNA1 develop malignant B cell lymphomas. Third, the expression of EBNA1 in Hodgkin cells enhances their ability to form tumors in nonobese diabetic-SCID mice . Fourth, EBV genomes lacking the EBNA1 gene are several thousand-fold less efficient at B cell immortalization than EBV genomes expressing EBNA1 . Fifth, interference with EBNA1 function in Burkitt’s lymphoma cells by overexpression of the EBNA1 DNA binding domain increased cell death, suggesting that EBNA1 normally provides a survival function for these cells.

 




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