Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human TransGlutaminase II Recombinant

TransGlutaminase II

accession:  P21980
   Size A     5ug   $ 70
   Size B      10ug  $160
   Size C      20ug  $ 700
Domain  : 
Gene  : 
Catalog no. :  RKP21980
Source:
Optimized DNA sequence encoding Human Interleukin-6 mature chain was expressed in Escherichia Coli

 
Molecular weight:
Recombinant human IL-6, generated by the proteolytic removal of the signal peptide
and propeptide, is a disulfide-linked homodimeric protein consisting of two 114 amino acid residue subunits. The molecule has a calculated molecular mass of approximately 26 kDa. rhIL-6 migrates as an approximately 32 kDa protein under non-reducing
conditions and as a 15 - 16 kDa protein under reducing conditions in SDS-PAGE.

 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of monkey 4MBr-5 cells was found to be in the range of 20.0-40.0 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MAEELVLERC DLELETNGRD HHTADLCREK LVVRRGQPFW LTLHFEGRNY EASVDSLTFS 

        70         80         90        100        110        120 
VVTGPAPSQE AGTKARFPLR DAVEEGDWTA TVVDQQDCTL SLQLTTPANA PIGLYRLSLE 

       130        140        150        160        170        180 
ASTGYQGSSF VLGHFILLFN AWCPADAVYL DSEEERQEYV LTQQGFIYQG SAKFIKNIPW 

       190        200        210        220        230        240 
NFGQFEDGIL DICLILLDVN PKFLKNAGRD CSRRSSPVYV GRVVSGMVNC NDDQGVLLGR 

       250        260        270        280        290        300 
WDNNYGDGVS PMSWIGSVDI LRRWKNHGCQ RVKYGQCWVF AAVACTVLRC LGIPTRVVTN 

       310        320        330        340        350        360 
YNSAHDQNSN LLIEYFRNEF GEIQGDKSEM IWNFHCWVES WMTRPDLQPG YEGWQALDPT 

       370        380        390        400        410        420 
PQEKSEGTYC CGPVPVRAIK EGDLSTKYDA PFVFAEVNAD VVDWIQQDDG SVHKSINRSL 

       430        440        450        460        470        480 
IVGLKISTKS VGRDEREDIT HTYKYPEGSS EEREAFTRAN HLNKLAEKEE TGMAMRIRVG 

       490        500        510        520        530        540 
QSMNMGSDFD VFAHITNNTA EEYVCRLLLC ARTVSYNGIL GPECGTKYLL NLNLEPFSEK 

       550        560        570        580        590        600 
SVPLCILYEK YRDCLTESNL IKVRALLVEP VINSYLLAER DLYLENPEIK IRILGEPKQK 

       610        620        630        640        650        660 
RKLVAEVSLQ NPLPVALEGC TFTVEGAGLT EEQKTVEIPD PVEAGEEVKV RMDLLPLHMG 

       670        680 
LHKLVVNFES DKLKAVKGFR NVIIGPA 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Interleukin-6 was lyophilized from a 0.2 μm filtered solution in 2.5% glycine, 0.5% sucrose, 0.01% Tween80, 5 mM Glutamic acid, pH 4.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

TransGlutaminase II



Related Publications:
distinct ph modulation for dual function of gh (transglutaminase ii)
J. Biochem., Mar 2005; 137: 407 - 413.
targeted inactivation of gh/tissue transglutaminase ii
J. Biol. Chem., Jun 2001; 276: 20673 - 20678.
phospholipase c1 is a guanine nucleotide exchanging factor for TransGlutaminase II (gh) and promotes 1b-adrenoreceptor-mediated gtp binding and intracellular calcium release
J. Biol. Chem., Feb 2001; 276: 5591 - 5597.
gtp binding and signaling by gh/TransGlutaminase II involves distinct residues in a unique gtp-binding pocket
J. Biol. Chem., Jun 2000; 275: 18259.
cardiac specific overexpression of TransGlutaminase II (gh) results in a unique hypertrophy phenotype independent of phospholipase c activation
J. Biol. Chem., Jul 1999; 274: 21291.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human TransGlutaminase II Recombinant
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $