Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human S100 Calcium Binding Protein A4 Recombinant

S100-A4

accession:  P26447
   Size A     2ug   $ 70
   Size B      10ug  $250
   Size C      50ug  $ 750
Domain  :  EF_HAND
Gene  :  S100A4
Catalog no. :  RKP26447
Source:
Optimized DNA sequence encoding Human S100A4 mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native human S100A4, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 12 kDa.

Recombinant s100-A4 is a disulfide-linked homodimer protein consisting of 332 amino acid residue subunits (containing a C-terminal IgG1 Fc tag), and migrates as an approximately 40 kDa protein under  reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Protein Sequence:
        10         20         30         40         50         60 
MACPLEKALD VMVSTFHKYS GKEGDKFKLN KSELKELLTR ELPSFLGKRT DEAAFQKLMS 

        70         80         90        100 
NLDSNRDNEV DFQEYCVFLS CIAMMCNEFF EGFPDKQPRK K 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant S100-A4 was lyophilized from a  0.2 μm filtered PBS solution and 1 mM DTT.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

S100A4

S100A4 is a calcium-binding protein related to the S100 family of proteins. It is known also as a growth- related protein, CAPL. It is expressed at high levels in different mouse metastatic cells and in normal tissues (thymus, spleen) and activated macrophages, T- cells, neutrophils, monocytes, macrophages .

Related Publications:
S100A4 and bone morphogenetic protein-2 codependently induce vascular smooth muscle cell migration via phospho–extracellular signal-regulated kinase and chloride intracellular channel 4
Circ. Res., Sep 2009; 105: 639 - 647.
autoimmune pancreatitis results from loss of tgfβ signalling in S100A4-positive dendritic cells
Gut, Sep 2009; 58: 1267 - 1274.
opposite roles of metastasin (S100A4) in two potentially tumoricidal mechanisms involving human lymphocyte protein tag7 and hsp70
PNAS, Aug 2009; 106: 13963 - 13967.
significance of S100A4 as a prognostic marker of lung squamous cell carcinoma
Anticancer Res, Jul 2009; 29: 2547 - 2554.
integrin 6β4 controls the expression of genes associated with cell motility, invasion, and metastasis, including S100A4/metastasin
J. Biol. Chem., Jan 2009; 284: 1484 - 1494.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human S100 Calcium Binding Protein A4 Recombinant
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $