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Human Thrombospondin-1 Recombinant

TSP-1

accession:  P07996
   Size A     2ug   $ 70
   Size B      10ug  $230
   Size C      100ug  $ 1900
Domain  :  EGF
Gene  :  THBS1,TSP1
Catalog no. :  RKP07996
Source:
Optimized DNA sequence encoding Human Thrombospondin mature chain was expressed in insect  cells.

 
Molecular weight:
Native human Thrombospondin, generated by the proteolytic removal of the signal peptide and propeptide, and has a calculated molecular mass of approximately 128 kDa.

Recombinant human TSP  is a disulfide-linked monomer protein consisting of 1152 amino acid residue subunits, and migrates due to glycosylation as an approximately 140 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>90%, as determined by SDS-PAGE and HPLC
 

 
Protein Sequence:
        10         20         30         40         50         60 
MGLAWGLGVL FLMHVCGTNR IPESGGDNSV FDIFELTGAA RKGSGRRLVK GPDPSSPAFR 

        70         80         90        100        110        120 
IEDANLIPPV PDDKFQDLVD AVRAEKGFLL LASLRQMKKT RGTLLALERK DHSGQVFSVV 

       130        140        150        160        170        180 
SNGKAGTLDL SLTVQGKQHV VSVEEALLAT GQWKSITLFV QEDRAQLYID CEKMENAELD 

       190        200        210        220        230        240 
VPIQSVFTRD LASIARLRIA KGGVNDNFQG VLQNVRFVFG TTPEDILRNK GCSSSTSVLL 

       250        260        270        280        290        300 
TLDNNVVNGS SPAIRTNYIG HKTKDLQAIC GISCDELSSM VLELRGLRTI VTTLQDSIRK 

       310        320        330        340        350        360 
VTEENKELAN ELRRPPLCYH NGVQYRNNEE WTVDSCTECH CQNSVTICKK VSCPIMPCSN 

       370        380        390        400        410        420 
ATVPDGECCP RCWPSDSADD GWSPWSEWTS CSTSCGNGIQ QRGRSCDSLN NRCEGSSVQT 

       430        440        450        460        470        480 
RTCHIQECDK RFKQDGGWSH WSPWSSCSVT CGDGVITRIR LCNSPSPQMN GKPCEGEARE 

       490        500        510        520        530        540 
TKACKKDACP INGGWGPWSP WDICSVTCGG GVQKRSRLCN NPTPQFGGKD CVGDVTENQI 

       550        560        570        580        590        600 
CNKQDCPIDG CLSNPCFAGV KCTSYPDGSW KCGACPPGYS GNGIQCTDVD ECKEVPDACF 

       610        620        630        640        650        660 
NHNGEHRCEN TDPGYNCLPC PPRFTGSQPF GQGVEHATAN KQVCKPRNPC TDGTHDCNKN 

       670        680        690        700        710        720 
AKCNYLGHYS DPMYRCECKP GYAGNGIICG EDTDLDGWPN ENLVCVANAT YHCKKDNCPN 

       730        740        750        760        770        780 
LPNSGQEDYD KDGIGDACDD DDDNDKIPDD RDNCPFHYNP AQYDYDRDDV GDRCDNCPYN 

       790        800        810        820        830        840 
HNPDQADTDN NGEGDACAAD IDGDGILNER DNCQYVYNVD QRDTDMDGVG DQCDNCPLEH 

       850        860        870        880        890        900 
NPDQLDSDSD RIGDTCDNNQ DIDEDGHQNN LDNCPYVPNA NQADHDKDGK GDACDHDDDN 

       910        920        930        940        950        960 
DGIPDDKDNC RLVPNPDQKD SDGDGRGDAC KDDFDHDSVP DIDDICPENV DISETDFRRF 

       970        980        990       1000       1010       1020 
QMIPLDPKGT SQNDPNWVVR HQGKELVQTV NCDPGLAVGY DEFNAVDFSG TFFINTERDD 

      1030       1040       1050       1060       1070       1080 
DYAGFVFGYQ SSSRFYVVMW KQVTQSYWDT NPTRAQGYSG LSVKVVNSTT GPGEHLRNAL 

      1090       1100       1110       1120       1130       1140 
WHTGNTPGQV RTLWHDPRHI GWKDFTAYRW RLSHRPKTGF IRVVMYEGKK IMADSGPIYD 

      1150       1160       1170 
KTYAGGRLGL FVFSQEMVFF SDLKYECRDP 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Thrombospondin-1 is presented as liquid formulation of 0.3M NaCl, 0.02M NaH2PO4  pH 6.0 .

 
Appearance:
Sterile solution.

 
Storage:

This cytokine should be stored in working aliquots at 2° - 8° C for one year.

Avoid freezing.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

Thrombospondin-1

TSP is an adhesive protein that can bind to many different cell types, including hematopoietic progenitor cells. It possesses a number of functional domains that serve as receptor recognizing regions. The amino-terminal heparin binding domain interacts with heparin, other glycosaminoglycans and glycolipids and likely recognizes specific cell surface proteoglycans. The central disulfide cross- linked region is responsible for high affinity binding to one of the CD antigens, CD36 . TSP has been demonstrated to bind to integrin-alpha-V , integrin-beta-3 and integrin-alpha-2b, integrin- beta-3. The carboxy-terminal region of TSP also contains at least one binding epitope for a cell receptor. TSP can interact with fibrinogen and fibronectin. TSP appears to be involved intimately in the regulation of cellular proliferation. It is expressed as an early response gene following treatment of cells with various mitogens and cytokines. It modulates the progression of cells through the Cell cycle. It augments the proliferative response to EGF. TSP inhibits endothelial cell chemotactic response to bFGF and reduces their proliferative response to serum and bFGF.

Related Publications:
regulation of Thrombospondin-1 by natural and synthetic progestins in human breast cancer cells
Endocr. Relat. Cancer, Sep 2009; 16: 809 - 817.
sjögren’s syndrome-like ocular surface disease in Thrombospondin-1 deficient mice
Am. J. Pathol., Sep 2009; 175: 1136 - 1147.
interactions among stalk modules of Thrombospondin-1
J. Biol. Chem., Aug 2009; 10.1074/jbc.M109.035089.
interactions among the epidermal growth factor-like modules of Thrombospondin-1
J. Biol. Chem., Aug 2009; 284: 22206 - 22212.
Thrombospondin-1 is a transcriptional repression target of prmt6
J. Biol. Chem., Aug 2009; 284: 21338 - 21346.




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