Human SDF-1 beta Recombinant
Categories: CXC chemokinesRecombinant Human Cytokines$70.00 – $2,700.00
Description
Accession
P48061
Source
Optimized DNA sequence encoding Human Stromal Cell-Derived Factor-1 beta mature chain was expressed in Escherichia Coli.
Molecular weight
Native human SDF-1 beta, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 8 kDa. Recombinant Stromal Cell-Derived Factor-1 beta is a monomer protein consisting of 72 amino acid residue subunits, migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Biological Activity
Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of-40 ng/ml.
Protein Sequence
MNAKVVVVLV LVLTALCLSD GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNKR FKM
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
SDF-1 beta was lyophilized from a0.2μm filtered concentrated (1.0mg/ml) solution inmM PB, pH7.0,mM NaCl.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
P15172
Biological Process
Molecular function
Molecular function
