Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Human SDF-1 beta Recombinant

SDF-1

accession:  P48061
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 2700
Domain  :  IL8
Gene  :  CXCL12
Catalog no. :  RKP480611
Source:
Optimized DNA sequence encoding Human Stromal Cell-Derived Factor-1 beta  mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native human SDF-1 beta, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 8 kDa.

Recombinant Stromal Cell-Derived Factor-1 beta is a disulfide-linked monomer protein consisting of 73 amino acid residue subunits, migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Determined by its ability to chemoattract human peripheral T cells activated with PHA and IL-2 using a concentration range of 5-40 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MNAKVVVVLV LVLTALCLSD GKPVSLSYRC PCRFFESHVA RANVKHLKIL NTPNCALQIV 

        70         80         90 
ARLKNNNRQV CIDPKLKWIQ EYLEKALNKR FKM 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
SDF-1 beta was lyophilized from a 0.2μm filtered concentrated (1.0mg/ml) solution in 20mM PB, pH 7.0, 150mM NaCl.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

SDF-1

SDF-1 alpha and SDF-1 beta are small cytokines belonging to the CXC- Chemokines. SDF-1 is identical with a chemokine reported to function as a pre-B-cell growth factor in the presence of IL7 and isolated originally from a murine bone marrow stromal cell line.Human SDF-1-alpha and SDF-1-beta are encoded by a single gene and arise by alternative splicing. SDF-1 acts on lymphocytes and monocytes but not neutrophils in vitro and is a highly potent chemoattractant for mononuclear cells in vivo. In addition. SDF-1 also induces intracellular actin polymerization in lymphocytes. SDF acts as a chemoattractant for human hematopoietic progenitor cells expressing CD34 giving rise to mixed types of progenitors, and more primitive types. The chemotactic response is inhibited by pertussis toxin. Chemotaxis of CD34(+) cells in response to SDF is increased by IL3 in vitro. SDF has been shown also to induce a transient elevation of cytoplasmic calcium in these cells.

Related Publications:
bone marrow stem and progenitor cell contribution to neovasculogenesis is dependent on model system with SDF-1 as a permissive trigger
Blood, Aug 2009; 10.1182/blood-2009-03-211342.
glycosaminoglycan mimetics inhibit SDF-1/cxcl12-mediated migration and invasion of human hepatoma cells
Glycobiology, Aug 2009; 10.1093/glycob/cwp130.
inhibition of the SDF-1-cxcr4 axis by the cxcr4 antagonist amd3100 suppresses the migration of cultured cells from atl patients and murine lymphoblastoid cells from htlv-i tax transgenic mice
Blood, Aug 2009; 10.1182/blood-2008-11-189308.
the cxcl12 (SDF-1)/cxcr4 axis is essential for the development of renal vasculature
J. Am. Soc. Nephrol., Aug 2009; 20: 1714 - 1723.
haematopoietic stem cell migration to the ischemic damaged kidney is not altered by manipulating the SDF-1/cxcr4-axis
Nephrol. Dial. Transplant., Jul 2009; 24: 2082 - 2088.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Rat SDF-1 alpha Recombinant
product_icon_8.jpg Human SDF-1 alpha Recombinant
product_icon_8.jpg Human SDF-1 beta Recombinant
product_icon_8.jpg Mouse SDF-1 alpha Recombinant
product_icon_8.jpg Mouse SDF-1 beta Recombinant
product_icon_8.jpg Rat Stromal Cell-Derived Factor-1 beta Recombinant
product_icon_8.jpg Human SDF-1 alpha Recombinant, Biotinylated
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $