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Human Neuregulin 1 beta Recombinant

NRG1

accession:  Q02297
   Size A     10ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 1350
Domain  :  EGF
Gene  :  NRG1
Catalog no. :  RKQ02297
Source:
Optimized DNA sequence encoding Human Neuregulin-1 beta EGF domain was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant human Neuregulin-1 beta EGF domain is a disulfide-linked monomeric protein consisting of 62 amino acid residue subunits, and migrates as an approximately 7 kDa protein under non-reducing and reducing conditions in SDS-PAGE.


 
Purity:
>96%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent proliferation of human MCF-7 cells was found to be <0.3ng/ml, corresponding to a specific activity of 2x 106Units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MSERKEGRGK GKGKKKERGS GKKPESAAGS QSPALPPRLK EMKSQESAAG SKLVLRCETS 

        70         80         90        100        110        120 
SEYSSLRFKW FKNGNELNRK NKPQNIKIQK KPGKSELRIN KASLADSGEY MCKVISKLGN

       130        140        150        160        170        180 
DSASANITIV ESNEIITGMP ASTEGAYVSS ESPIRISVST EGANTSSSTS TSTTGTSHLV 

       190        200        210        220        230        240 
KCAEKEKTFC VNGGECFMVK DLSNPSRYLC KCPNEFTGDR CQNYVMASFY KHLGIEFMEQ 

       250        260        270        280        290        300 
KRVLTITGIC IALLVVGIMC VVAYCKTKKQ RKKLHDRLRQ SLRSERNNMM NIANGPHHPN
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Neuregulin-1 was lyophilized from a 0.2 μm filtered 10mM PB, pH 7.0,
containing 2% mannitol, 0.5% HSA.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Neuregulin-1

Neuregulins (NDF, heregulin, GGF ARIA, or SMDF) are EGF- like growth and differentiation factors that signal through tyrosine kinase receptors of the ErbB family.The ErbB2 and ErbB4 receptors cooperate in transmission of neuregulin-1 signals in the heart, whereas ErbB2 and ErbB3 cooperate in neural crest cells.

Related Publications:
the tyrosine phosphatase shp2 (ptpn11) directs Neuregulin-1/erbb signaling throughout schwann cell development
PNAS, Sep 2009; 10.1073/pnas.0904336106.
Neuregulin-1β is associated with disease severity and adverse outcomes in chronic heart failure
Circulation, Jul 2009; 120: 310 - 317.
the relationship among neuregulin 1–stimulated phosphorylation of akt, psychosis proneness, and habituation of arousal in nonclinical individuals
Schizophr Bull, Jun 2009; 10.1093/schbul/sbp063.
sensory axon-derived Neuregulin-1 is required for axoglial signaling and normal sensory function but not for long-term axon maintenance
J. Neurosci., Jun 2009; 29: 7667 - 7678.
downregulation of neuregulin 1 in retinal ganglion cells in experimental glaucoma
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 2075.




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