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Toxoplasma Gondii SAG1 protein Recombinant

TOXGO SAG1

accession:  Q27298
   Size A       $
   Size B      0.5mg  $800
   Size C      1mg  $ 1100
Domain  : 
Gene  :  P30
Catalog no. :  RKQ27298
Source:
DNA sequence encoding immunodominant  fragment of Toxoplasma SAG1(p30) protein was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant TOXGO SAG1(p30) encodes the immunodominant sequences of the Toxoplasma Dense Granule protein.

Recombinant SAG1 contains an N terminal GST purification tag and migrates as an approximately 43 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE.
 
Applications:

Toxoplasma SAG1 antigen is suitable for ELISA and Western blots, excellent antigen for detection of Toxoplasma with minimal specificity problems.

 

 Specificity:
Immunoreactive with sera of Toxopalsma-infected individuals.
 
Presentation:
Lyophilized from 50mM Tris-HCl, 60mM NaCl and 50% glycerol.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This  product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

SAG1

Recent studies indicate that SAG1 may play an important role in invasion of host cells. Unlike surface antigens of other protozoa, such as plasmodia and trypanosomes, SAG1 is a nonvariant antigen which is well conserved immunologically and at the sequence level. SAG1 elicits a robust immune response in infected animals, including humans, and immunization with purified SAG1 protects mice from a lethal parasite challenge. This immunity appears to be primarily mediated by CD8+ cells specific for SAG1

Related Publications:
evaluation of nested pcr targeting b1 gene with four npcrs (targeting the b1, sag2 and SAG1 gene) for detection of toxoplasma gondii genome in intra ocular fluids from toxoplasma retinochoroiditis patients in a tertiary eye hospital, chennai, india
Invest. Ophthalmol. Vis. Sci., Apr 2009; 50: 857.
reverse enzyme-linked immunosorbent assay using monoclonal antibodies against SAG1-related sequence, sag2a, and p97 antigens from toxoplasma gondii to detect specific immunoglobulin g (igg), igm, and iga antibodies in human sera
Clin. Vaccine Immunol., Aug 2008; 15: 1265 - 1271.
azurin-like protein blocks invasion of toxoplasma gondii through potential interactions with parasite surface antigen SAG1
Antimicrob. Agents Chemother., Feb 2008; 52: 402 - 408.
the SAG1 toxoplasma gondii surface protein is not required for acute ocular toxoplasmosis in mice
Infect. Immun., Apr 2007; 75: 2079 - 2083.
conformationally correct expression of membrane-anchored toxoplasma gondii SAG1 in the primitive protozoan giardia duodenalis
Infect. Immun., Feb 2002; 70: 1014 - 1016.




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