//Biotinylated SARS-COV-2 Spike S1 (Omicron B.529) Protein Recombinant

Biotinylated SARS-COV-2 Spike S1 (Omicron B.529) Protein Recombinant

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$542.00$1,805.00

SKU: KT7VFBHHB

Description

Accession
QHO60594
Source
DNA sequence encoding SARS-COV-2 Spike S1 (Omicron B.1.1.529) including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529) is a protein consisting of amino acid residues [Gln14-Arg683(A67V, HV69-70del, T95I, G142D, VYY143-145del, N211del, L212I, ins214EPE, G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H, T547K, D614G, H655Y, N679K, P681H)] ,The protein has a predicted MW of 77.94 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant SARS-COV-2 Spike S1 (Omicron B.1.1.529) was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

Methods

Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein Tris-Bis PAGE

  • Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein SEC-HPLC

  • The purity of Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) is greater than 95% as determined by SEC-HPLC.

Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) Protein ELISA Data

  • Immobilized Biotinylated Biotinylated SARS-COV-2 Spike S1 (Omicron B.1.1.529) , His Tag at 1μg/ml (100μl/Well) on streptavidin (5μg/ml) precoated plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 0.25μg/ml determined by ELISA.
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