Description
Accession
P59595
Source
DNA sequence encoding immunodominant fragment of SARS Nucleocapsid protein was expressed in Escherichia Coli.
Molecular weight
Recombinant SARS N encodes the N terminal immunodominant sequences of the SARS Nucleocapsid protein. Recombinant SARS N contains an N terminal GST purification tag and migrates as an approximately 33 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE.
Applications
SARS antigen is suitable for ELISA and Western blots, excellent antigen for detection of SARS with minimal specificity problems.
Presentation
Lyophilized from 50mM Tris-HCl, 60mM NaCl and 50% glycerol.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Molecular function
Molecular function
Methods
Recombinant SARS (hCov) N Protein PAGE
- The recombinant SARS N protein migrates as 30kDa under reducing conditions
