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Mouse TNF-alpha Recombinant

TNFA

accession:  P06804
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  TNF
Gene  :  TNFA
Catalog no. :  RKP06804

Source:
Optimized DNA sequence encoding Mouse Tumor Necrosis Factor-alpha  extracellular domain was expressed in Escherichia Coli.

 
Molecular weight:

Native Mouse Tumor Necrosis Factor-alpha is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately  17kDa.

Recombinant Mouse TNF is a disulfide-linked monomer  protein, consisting of 157 amino acids and migrates as an approximately 17 kDa protein under reducing conditions.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined cytolysis of murine L929 cells in the presence of Actinomycin D is ≤ 0.05 ng/ml, corresponding to a specific activity of ≥ 3 x 107 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MSTESMIRDV ELAEEALPQK MGGFQNSRRC LCLSLFSFLL VAGATTLFCL LNFGVIGPQR 

        70         80         90        100        110        120 
DEKFPNGLPL ISSMAQTLTL RSSSQNSSDK PVAHVVANHQ VEEQLEWLSQ RANALLANGM 

       130        140        150        160        170        180 
DLKDNQLVVP ADGLYLVYSQ VLFKGQGCPD YVLLTHTVSR FAISYQEKVN LLSAVKSPCP 

       190        200        210        220        230 
KDTPEGAELK PWYEPIYLGG VFQLEKGDQL SAEVNLPKYL DFAESGQVYF GVIAL 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse TNF alpha was lyophilized from 0.2 μm filtered PBS solution, pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

TNF-alpha

TNF is secreted by macrophages, monocytes, neutrophils, T-cells, NK- cells following their stimulation by bacterial lipopolysaccharides. Cells expressing CD4 secrete TNF- alpha while CD8(+) cells secrete little or no TNF- alpha. Stimulated peripheral neutrophilic granulocytes but also unstimulated cells and also a number of transformed cell lines, astrocytes, microglial cells, smooth muscle cells, and fibroblasts also secrete TNF. Human milk also contains this factor. The synthesis of TNF-alpha is induced by many different stimuli including interferons, IL2, GM-CSF, SP, Bradykinin, Immune complexes, inhibitors of cyclooxygenase and PAF (platelet activating factor). Human TNF-alpha is a non- glycosylated protein of 17 kDa and a length of 157 amino acids. Murine TNF- alpha is N-glycosylated. Homology with TNF-beta is approximately 30 %. TNF- alpha forms dimers and trimers.

Related Publications:
tak1-mediated serine/threonine phosphorylation of epidermal growth factor receptor via p38/extracellular signal-regulated kinase: nf-b-independent survival pathways in tumor necrosis factor alpha signaling
Mol. Cell. Biol., Oct 2009; 29: 5529 - 5539.
tumor necrosis factor--mediated regulation of the inositol 1,4,5-trisphosphate receptor promoter
J. Biol. Chem., Oct 2009; 284: 27557 - 27566.
tnf- inhibition attenuates adverse myocardial remodeling in a rat model of volume overload
Am J Physiol Heart Circ Physiol, Oct 2009; 297: H1462 - H1468.
tnf inhibits apoptotic cell clearance in the lung, exacerbating acute inflammation
Am J Physiol Lung Cell Mol Physiol, Oct 2009; 297: L586 - L595.
association of tnf- gene polymorphisms with systemic lupus erythematosus in taiwanese patients
Lupus, Oct 2009; 18: 974 - 979.




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